Qiagen/QuantiNova SYBR®绿色RT-PCR试剂盒/适用于100 x 20和micro；l反应：1毫升QuantiNova SYBR绿色RT-PCR主混合物，20和微量；l QuantiNova SYBR绿色RT混合，20微米；l内部控制RNA，500µ；l-黄色模板稀释缓冲液
市场价：
¥4780.00
美元价：
2868.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
QuantiNova SYBR® Green RT-PCR Kit
Print
For one-step qRT-PCR using SYBR Green I for gene expression analysis
- Unique two-phase hot-start procedure for room-temperature setup
- Visual pipetting control, resulting in fewer pipetting errors
- Internal control for positive in-process verification of successful RT-PCR
- Accurate quantification over several logs of template
- Ultrafast, universal protocol for all standard and fast cyclers
The QuantiNova SYBR Green RT-PCR Kit enables sensitive quantification of RNA targets by real-time one-step PCR using SYBR Green I detection. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. The combination of a unique two-phase hot-start and PCR buffer system in the ready-to-use master mix allows room-temperature setup and ensures highly sensitive qRT-PCR on any real-time cycler. The QuantiNova Internal Control RNA can be used to test successful reverse transcription and amplification. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. What"s more, the visual indicator reduces pipetting error when performing ultrafast RT-PCR in less than one hour.
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Cat No./ID:208152 QuantiNova SYBR Green RT-PCR Kit (100) $239.00 For 100 x 20 µl reactions: 1 ml QuantiNova SYBR Green RT-PCR Master Mix, 20 µl QuantiNova SYBR Green RT Mix, 20 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 250 µl ROX Reference Dye, 1.9 µl RNase-Free Water |
Cat No./ID:208154 QuantiNova SYBR Green RT-PCR Kit (500) $929.00 For 500 x 20 µl reactions: 3 x 1.7 ml QuantiNova SYBR Green RT-PCR Master Mix, 100 µl QuantiNova SYBR Green RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water |
Cat No./ID:208156 QuantiNova SYBR Green RT-PCR Kit (2500) $3,722.00 For 2500 x 20 µl reactions: 15 x 1.7 ml QuantiNova SYBR Green RT-PCR Master Mix, 5 x 100 µl QuantiNova SYBR Green RT Mix, 2 x 100 µl Internal Control RNA , 5 x 500 µl Yellow Template Dilution buffer, 5 x 1 ml ROX Reference Dye, 10 x 1.9 µl RNase-Free Water |
Cat No./ID:QT02589307 QuantiNova IC SYBR Green Assay QuantiTect Primer Assay for SYBR-based detection of QuantiNova Internal Control RNA; available via GeneGlobe (sufficient for approx. 500 x 20 µl rxn). For use with QuantiNova SYBR Green PCR Kit or QuantiNova SYBR Green RT-PCR Kit |
Product Details
Performance
The QuantiNova SYBR Green RT-PCR Kit uses a two-phase hot-start procedure (see figure “Principle of the novel QuantiNova two-phase hot-start mechanism”). This includes heat-mediated activation of both the HotStaRT-Script reverse transcriptase at 50°C and the PCR polymerase at 95°C. At low temperatures the HotStaRT-Script forms a complex with an RT-Blocker molecule, leading to inactivation. At 50°C, the complex dissociates and the active HotStaRT-Script enzyme is released. The QuantiNova DNA Polymerase is kept in an inactive state by the QuantiNova Antibody and a novel additive, QuantiNova Guard, which stabilizes the complex. This improves the stringency of the hot-start procedure and prevents extension of non-specifically annealed primers and primer–dimers. Within2 minutes of raising the temperature to 95°C, the QuantiNova Antibody and QuantiNova Guard are denatured and the QuantiNova DNA Polymerase is activated, enabling PCR amplification. This unique two-phase hot-start enables reaction setup to remain stable for at least 2 hours at room temperature, preventing the creation of artifacts and also facilitating automated reaction setup.The newly developed internal control is a defined transcript (RNA molecule) that can simply be added to your samples and transcribed into cDNA. It is intended to report instrument or chemistry failures, errors in assay setup or the presence of inhibitors. Since the internal control behaves comparably to real transcripts, it can be used to confirm successful reverse transcription and amplification. The master mix supplied with the QuantiNova SYBR Green RT-PCR Kit contains an inert blue dye that does not interfere with the reverse transcription or real-time PCR, but increases visibility in the tube or well. The QuantiNova Yellow Template Dilution Buffer contains an inert yellow dye. When the template nucleic acid, diluted with the QuantiNova Yellow Template Dilution Buffer, is added to the master mix, the color of the solution changes from blue to green, providing a visual indication of correct pipetting and reaction setup (see figure “Accurate reaction setup indicated by the built-in pipetting control”). The QuantiNova DNA Polymerase and the unique composition of the RT-PCR buffer provide sensitive quantification of low-copy RNA targets, as well as accurate quantification over a wide linear range on any common cycler.
Principle
The QuantiNova SYBR Green RT-PCR Kit contains an optimized, ready-to-use master mix for highly specific and sensitive real-time quantification of RNA targets using fluorescent dye SYBR Green I.The kit comes with a unique PCR buffer that contains a balanced combination of K+ and NH4+ ions, which promotes specific primer annealing, enabling high specificity and sensitivity. In addition, the HotStaRT-Script enables cDNA synthesis from a wide range of RNA template amounts, while the QuantiNova DNA Polymerase provides a stringent hot-start procedure, preventing the formation of nonspecific products. The unique two-phase hot-start procedure allows reaction setup to remain stable for up to two hours at room temperature supporting automated reaction setup.The built-in visual control allows you to check whether template has been added to the reaction and therefore prevents pipetting errors during reaction setup. Detecting variations in cDNA synthesis allows you to check the reproducibility of your results. The newly developed internal control is a defined transcript (RNA molecule) that can be optionally added to your samples and transcribed into cDNA. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors.
Procedure
The QuantiNova SYBR Green RT-PCR Kit overcomes the need for optimization of reaction conditions, which can be tedious and time consuming. Simply add primers, RNA template and RT-Mix to the ready-to-use SYBR Green RT-PCR master mix and start the reaction. Follow the protocol in the QuantiNova SYBR Green RT-PCR Kit Handbook to get fast and reliable results on any real-time cycler.
Applications
The QuantiNova SYBR Green RT-PCR Kit can be used for gene expression analysis of RNA targets on any real-time cycler. This includes the Rotor-Gene Q and instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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