FastLane Cell cDNA Kit
- From cells to cDNA in only 4 steps and under 45 minutes
- Sensitive detection of even low-abundance transcripts
- Easy parallel processing of several samples
- Integrated gDNA removal allows detection of RNA only
- No need to design RNA-specific primers or probes
The FastLane Cell cDNA Kit provides a fast and simple procedure for preparing first-strand cDNA directly from cultured cells without RNA purification. The kit is supplied with wash and lysis buffers for preparing lysates and stabilizing RNA, gDNA Wipeout Buffer for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis. The FastLane Cell cDNA Kit is ideal for experiments requiring rapid, high-throughput gene expression analysis.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:215011 FastLane Cell cDNA Kit (50) $675.00 Buffer FCW, Buffer FCP, and components for 50 x 20 µl reverse-transcription reactions (gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, and RNase-Free Water) |
Product Details
The FastLane Cell cDNA Kit uses FastLane technology to prepare cDNA directly from cultured cells in just 45 minutes. To ensure only RNA is detected in real-time RT-PCR, novel gDNA Wipeout Buffer is used to eliminate genomic DNA (see figure "Effective elimination of genomic DNA contamination"). Time and effort are saved as there is no need to design RNA-specific primers or probes.
When the FastLane Cell cDNA Kit is used together with optimized QuantiTect, QuantiFast, or Rotor-Gene Kits for real-time RT-PCR, several cell samples can be easily processed and analyzed within a few hours. This allows a snapshot of several transcripts in experiments such as validation of siRNA-mediated gene knockdown (see figure "Successful analysis of CDC2 knockdown").
The FastLane Cell cDNA Kit is part of the comprehensive range of FastLane Kits, which speed up and streamline the gene expression analysis workflow.
With the FastLane Cell cDNA Kit, cDNA is obtained from cultured cells in just 45 minutes in a 4-step procedure: cell wash; cell lysis with integrated RNA stabilization; genomic DNA elimination; and cDNA synthesis. The synthesized cDNA is ready to use in real-time two-step RT-PCR.
The kit contains sufficient reagents for preparing cDNA from two 24-well cell-culture plates or one 48-well cell-culture plate. If working with a 96-well cell-culture plate, an additional QuantiTect Reverse Transcription Kit (50) needs to be purchased.
cDNA prepared with the FastLane Cell cDNA Kit can be analyzed by real-time two-step RT-PCR using one of the following QuantiTect Kits:
- SYBR® Green detection: QuantiTect SYBR® Green PCR Kit
- Probe detection: QuantiTect Probe PCR Kit
- Multiplex probe detection: QuantiTect Multiplex PCR Kits
Alternatively, fast real-time two-step RT-PCR can be carried out when the FastLane Cell cDNA Kit is used in combination with a QuantiFast Kit:
- SYBR® Green detection: QuantiFast SYBR® Green PCR Kit
- Probe detection: QuantiFast Probe PCR Kits
- Multiplex probe detection: QuantiFast Multiplex PCR Kits
Ultrafast real-time two-step RT-PCR can be achieved when cDNA prepared with the FastLane Cell cDNA Kit is analyzed on the Rotor-Gene Q cycler using one of the following dedicated Rotor-Gene Kits:
- SYBR® Green detection: Rotor-Gene SYBR® Green PCR Kit
- Probe detection: Rotor-Gene Probe PCR Kit
- Multiplex probe detection: Rotor-Gene Multiplex PCR Kit
QuantiTect and QuantiFast Kits are compatible with various real-time cyclers, including instruments from Applied Biosystems.
The FastLane Cell cDNA Kit is suitable for experiments which require a snapshot of individual transcript levels, such as:
- Validation of siRNA-mediated gene knockdown
- Evaluation of drug effects
- Detection of gene regulation
Specifications
Features | Specifications |
| Applications | Real-time, two-step RT-PCR, gene expression analysis directly from cells |
| Enzyme activity | Reverse transcription |
| Mastermix | No |
| Reaction type | cDNA production, DNA digestion |
| Real-time or endpoint | Real-time, two-step RT-PCR |
| Sample/target type | Cultured cells |
| Single or multiplex | Single |
| With/without hotstart | Without hotstart |
Product Resources
Customers who bought these products also bought
- Cat No./ID:399911
AdnaMag-S
For 8 tubes, 1.5 ml$708.00Add To Cart - Cat No./ID:32915
N-Terminus pQE Vector Set
25 µg each: pQE-9, pQE-30, pQE-31, pQE-32, pQE-40$718.00Add To Cart - Cat No./ID:19505
QIAvac HiSpeed LS
QIAvac HiSpeed LS$5,579.00Add To Cart - Cat No./ID:1070498
Buffer TCL, 2x (8.5 ml)
8.5 ml of 2x lysis buffer for TurboCapture mRNA Kits$77.70Add To Cart - Cat No./ID:218761
miScript Plant RT Kit (50)
For fifty 20 µl first strand cDNA synthesis reactions; 10x miScript Ligation Buffer, 10x miScript Plant Adaptor, 2x miScript Ligation Activator, miScript Plant RNA Ligase, 5x miScript RT Buffer, 10x miScript Plant RT Nucleics, miScript Plant Reverse Transcriptase, RNase-Free Water$520.00Add To Cart - Cat No./ID:399921
AdnaMag-L
For 8 tubes, 15 ml$708.00Add To Cart - Cat No./ID:180773
QIAseq Stranded mRNA Select Kit (24)
For mRNA enrichment and strand-specific RNA-seq library preparation of 24 samples: Kits include magnetized reaction cleanup beads and sample index adapters for Illumina NGS platforms
$1,247.00Add To Cart - Cat No./ID:204143
QuantiTect SYBR Green PCR Kit (200)
For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$459.00Add To Cart - Cat No./ID:36113
Ni-NTA Magnetic Agarose Beads (6 x 1 ml)
6 x 1 ml nickel-charged magnetic agarose beads (5%suspension)$703.00Add To Cart - Cat No./ID:1014630
Buffer AP1 (200/250),KG
Buffer AP1 (200/250),KG$87.60Add To Cart - Cat No./ID:205411
QuantiNova Reverse Transcription Kit (50)
For 50 x 20 µl reactions: 100 µl 8x gDNA Removal Mix, 50 µl Reverse Transcription Enzyme, 200 µl Reverse Transcription Mix (containing RT primers), 100 µl Internal Control RNA, 1.9 ml RNase-Free Water$332.00Add To Cart - Cat No./ID:208256
QuantiNova Probe PCR Kit (2500)
For 2500 x 20 µl reactions: 15 x 1.7 ml 2x QuantiNova Probe PCR Master Mix, 5 x 1 ml QN ROX Reference Dye, 5 x 500 µl QuantiNova Yellow Template Dilution Buffer, 5 x 1.9 ml Water$1,638.00Add To Cart - Cat No./ID:74124
RNeasy Protect Mini Kit (50)
RNAprotect TissueReagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
$389.00Add To Cart - Cat No./ID:20912
Buffer QX1 (500 ml)
500 ml Solubilization and Binding Buffer (with pH indicator)$107.00Add To Cart - Cat No./ID:330411
RT² HT First Strand Kit (96)
RT2 HT First Strand Kit$594.00Add To Cart - Cat No./ID:381575
Investigator ESSplex SE QS Kit (100)
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water$2,196.00Add To Cart - Cat No./ID:74126
RNeasy Protect Mini Kit (250)
RNAprotect TissueReagent (250 ml), 250RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
$1,681.00Add To Cart - Cat No./ID:27191
QIAprep 96 Turbo Miniprep Kit (4)
For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; S-Blocks, reagents, buffers, collection microtubes (1.2 ml), caps
$1,168.00Add To Cart - Cat No./ID:208054
QuantiNova SYBR Green PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova SYBR Green PCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer, 1 ml QN ROX Reference Dye, 1.9 ml Water$429.00Add To Cart - Cat No./ID:205313
QuantiTect Rev. Transcription Kit (200)
For 200 x 20 µl reactions: 4 x 100 µl 7x gDNA Wipeout Buffer, 4 x 50 µl Quantiscript Reverse Transcriptase, 4 x 200 µl 5x Quantiscript RT Buffer, 4 x 50 µl RT Primer Mix, 4 x 1.9 ml RNase-Free Water$1,300.00Add To Cart
