Rotor-Gene SYBR® Green PCR Kit
For ultrafast, real-time PCR and two step RT-PCR using SYBR Green on Rotor-Gene cyclers
- Specific and sensitive detection of even low-copy targets
- Accurate detection of a wide range of template amounts
- Specially formulated master mix for reliable results without optimization
- Guaranteed performance combined with QuantiTect Primer Assays
The Rotor-Gene SYBR Green PCR Kit is designed for use with the Rotor-Gene Qand other Rotor-Gene cyclers, providing ultrafast, highly specific quantification of gDNA and cDNA targets withquantitative real-time PCR ortwo-step RT-PCR using SYBR Green I detection. Outstanding qPCR performance is achieved through the combination of a specially optimized master mix and the unique Rotor-Gene cycler. For convenience, the master mix can be stored at 2–8°C.
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Cat No./ID:204074 Rotor-Gene SYBR Green PCR Kit (400) $471.00 For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water |
Cat No./ID:204076 Rotor-Gene SYBR Green PCR Kit (2000) $1,608.00 For 2000 x 25 µl reactions: 25 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 20 ml RNase-Free Water |
Product Details
The Rotor-Gene SYBR Green PCR Kit is well suited for use in gene expression analysis of cDNA targets (see figures "Highly specific and sensitive detection" and "Reliable and specific detection down to 10 copies").
When QuantiTect Primer Assays are used together with the Rotor-Gene SYBR Green PCR Kit, highly sensitive quantification of specific PCR products is achieved without the need for optimization (see table).
| QIAGEN | Supplier AII | |||
| CT | Mean deviation | CT | Mean deviation | |
|---|---|---|---|---|
| BAX (BCL2-associated X protein) | 24.84 | 0.05 | 29.57 | 0.46 |
| BCL2 (apoptosis gene) | 26.96 | 0.05 | 32.83 | 0.29 |
| MYC (proto-oncogene) | 28.42 | 0.21 | 35.26 | 0.72 |
| b-Actin (housekeeping gene) | 20.24 | 0.03 | 24.39 | 0.12 |
The Rotor-Gene SYBR Green PCR Kit enables rapid and reliable real-time PCR quantification on the Rotor-Gene Q without the need for optimization of reaction and cycling conditions. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. Highly specific amplification is assured through a balanced combination of K+ and NH4+ ions, which promote specific primer annealing, enabling high PCR specificity and sensitivity (see figure "Specific primer annealing"). Fast cycling without compromising performance is achieved using Q-Bond, a novel PCR additive that enables cycler run times of as low as 45 minutes (see figure "Fast primer annealing").
| Component | Features | Benefits |
|---|---|---|
| HotStarTaq Plus DNA Polymerase | 5 min activation at 95ºC | Set up of qPCR reactions at room temperature |
| Rotor-Gene SYBR Green PCR Buffer | Balanced combination of NH4+ and K+ ions | Specific primer annealing ensures reliable qPCR results |
| Unique Q-Bond additive | Faster PCR run times enable faster results and more reactions per day | |
| SYBR Green I dye | Yields a strong fluorescent signal upon binding double-stranded DNA | Highly sensitive quantification |
The Rotor-Gene SYBR Green PCR Master Mix eliminates the need for optimization of reaction and cycling conditions. Simply add DNA template DNA and primers to the master mix and program the cycler. Instructions are provided in the detailed handbook supplied with the kit.
For gene expression analysis using real-time two-step RT-PCR, the combination of the Rotor-Gene SYBR Green PCR Kit, QuantiTect Reverse Transcription Kit, QuantiTect Primer Assays, and Rotor-Gene Q provides a complete, ready-to-run solution. The QuantiTect Reverse Transcription Kit delivers fast cDNA synthesis in just 20 minutes with integrated removal of genomic DNA contamination. QuantiTect Primer Assays are bioinformatically validated primer sets for any gene from human, mouse, rat, and many other species. Assays can be easily ordered online at the GeneGlobe Web portal.
The Rotor-Gene SYBR Green PCR Kit provides rapid real-time quantification of cDNA and gDNA targets on the Rotor-Gene Q. The Kits are also compatible with the Rotor-Gene 3000 and the Rotor-Gene 6000.
For ultrafast, one-step qRT-PCR gene expression analysis of RNA targets using SYBR Green I on Rotor-Gene cyclers, use the Rotor-Gene SYBR Green RT-PCR Kit.
Specifications
Features | Specifications |
| Applications | Real-Time quantification of genomic DNA or cDNA targets |
| Description | For ultrafast quantitative real-time PCR and two-step RT-PCR using SYBR Green I on Rotor-Gene cyclers |
| Reaction type | Real-time PCR and two-step RT-PCR |
| Real-time or endpoint | Real-time |
| Sample/target type | DNA, cDNA |
| Single or multiplex | Single |
| SYBR Green I or sequence-specific probes | SYBR Green I |
| Thermal cycler | Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000 |
| With or without ROX | Without ROX dye |
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