Qiagen/QIAseq UPX 3’ Transcriptome Kits/For indexing up to 12 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 12 x 96-well plates (1152 cells/wells) or 12 x 384-well
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¥20660.00
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12396.00
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3392242852
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4000-520-616
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商品介绍
QIAseq UPX 3’ Transcriptome Kits
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For high-throughput 3" transcriptome NGS from ultralow amounts of RNA
- Start with 1–100 cells or 10 pg to 1 ng of isolated RNA
- LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
- UMIs eliminate library amplification bias for accurate gene expression
- UPX tagging allows 4608–18,432 samples per single sequencing lane
- Includes cloud-based read alignment and single-cell or low-input analysis
QIAseq UPX 3" Transcriptome Kits enable high-throughput gene expression analysis from single cells and previously isolated RNA. Each kit is enough for 96 or 384 libraries, with kit format comprising of either a 96-well multi-break plates or 384-well single use plates with reverse transcription primers already lyophilized in the bottom of the plate for ease of use. To build libraries for RNA-seq, simply add 1 to 100 cells into each well or 10 pg to 1 ng of RNA to reconstitute a reverse transcription reaction. After cDNA synthesis, combine each plate into a single tube to complete the library preparation. All reagents necessary for starting with cells (such as lysis reagents) or RNA are included in the kit. Want to try this solution for the first time? Request a quote for a trial kit.
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Cat No./ID:333074 QIAseq UPX 3" Trans. 12-Index (48) $1,033.00 For indexing up to 12 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 12 x 96-well plates (1152 cells/wells) or 12 x 384-well plates (4608 cells/wells) per lane |
Cat No./ID:333075 QIAseq UPX 3" Trans. 48-Index (192) $2,067.00 For indexing up to 48 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 48 x 96-well plates (4608 cells/wells) or 48 x 384-well plates (18,432 cells/wells) per lane |
Cat No./ID:333088 QIAseq UPX 3" Transcriptome Kit (96) $2,959.00 For 3" gene expression analysis from cells or mRNA of 8–96 samples at one time; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized in a 96-well plate which can be easily separated for processing 8–96 samples in parallel (this format is ideal for both small and large sample sizes) |
Cat No./ID:333089 QIAseq UPX 3" Transcriptome Kit (96-M) $8,996.00 For 3" gene expression analysis from cells or mRNA of up to 384 samples; each kit contains cell lysis solution, buffers, oligos and enzymes in a 96-well plate. The increased volumes allow for automation or manual pipetting or where custom protocols are necessary. |
Cat No./ID:333090 QIAseq UPX 3" Transcriptome Kit (384) $8,996.00 For 3" gene expression analysis from cells or mRNA of 384 samples simultaneously; each kit contains cell lysis solution, buffers, oligos and enzymeslyophilized on a 384-well plate for processing 384 samples in parallel (this format is best suited for large samples numbers of at least 384 samples) |
QIAseq UPX 3’ Transcriptome Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAseq UPX 3" Transcriptome Kit presents a unique advantage where each cell is tagged with a unique ID (up to 384 different IDs) and each RNA molecule is tagged with a Unique Molecular Index (UMI) during reverse transcription. Following reverse transcription with integrated template switching, all individually tagged cDNAs can be combined, which enables all subsequent library construction steps to be performed in a single tube – saving significant time and library prep costs. QIAseq UPX 3’ Transcriptome Kits provide a convenient, streamlined and reproducible library construction workflow starting with as little as 10 pg total RNA (see figures "Streamlined and Reproducible Workflow Starting from 10 pg to 1 ng of Total RNA", "Consistency of Detection with QIAseq UPX 3’ Transcriptome Kitsand "Library QC and Quantification").
Principle
QIAseq UPX 3" Transcriptome Kits enable Sample to Insight, high-throughput NGS of polyadenylated RNAs from single cells on Illumina NGS instruments. Kits are intended for library construction and analysis of cell pellets (up to 100 cells) and purified RNA (10 pg to 1 ng).QIAseq UPX 3" Transcriptome Kits present an innovative advantage in that during reverse transcription, each cell is tagged with a unique ID (up to 384 different IDs) and each RNA molecule is tagged with a Unique Molecular Index (UMI). Following reverse transcription with integrated template switching, all individually tagged cDNAs can be combined, which enables all subsequent library construction steps to be performed in a single tube. This prevents sample mixup, saves substantial time and dramatically reduces library prep costs. During subsequent amplification and library construction up to 48 different sample IDs can be assigned. Together, the combination of cell IDs and sample IDs enables up to 18,432 libraries to be sequenced together. QIAseq UPX data analysis enables primary mapping, single-cell clustering analysis and differential expression analysis.
Procedure
Innovative, optimized workflow
The QIAseq UPX 3" Transcriptome Kit defines a new generation of high-throughput NGS technologies in QIAGEN’s Sample to Insight workflow.Purified RNA or single cells are first reverse transcribed and each RNA molecule is given a Unique Molecular Index (UMI) and well-specific IDs are assigned (up to 384 wells; cell IDs). Following reverse transcription with integrated template switching, all cDNAs are combined, enabling simplified library construction steps to be performed in a single tube.During subsequent amplification and library construction, up to 48 different library indices (sample IDs) can be assigned. The combination of cell IDs and sample IDs enables up to 18,432 libraries to be sequenced simultaneously (see figures "Innovative and Optimized QIAseq UPX 3" Transcriptome Kit Workflow" and "QIAGEN’s Sample to Insight QIAseq UPX 3" Transcriptome Workflow").Convenient GeneGlobe data analysis
QIAseq UPX cloud-based data analysis is available via the GeneGlobe Data Analysis Center and provides read alignments, UMI and sample de-multiplexing, with final single-cell or low-input gene expression analysis.Applications
- High-throughput screening applications (e.g., toxicology research)
- Single-cell analysis
- Differential gene expression analysis
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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