Qiagen/QIAseq UPX 3’ Targeted RNA Panels/Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed)
/333041
/333041
市场价：
¥14460.00
美元价：
8676.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QIAseq UPX 3’ Targeted RNA Panels
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For ultraplex (UPX) gene expression analysis using 3" RNA-seq from single cells and low-input samples
- Target up to 1000 genes using a cost-effective, time-saving single-tube library prep
- LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
- UMIseliminate library amplification bias for accurate gene expression
- Cell tagging and sample indexing enables simultaneous sequencing of up to 147,456 targeted libraries
- Includes cloud-based read alignment and single-cell or low-input analysis
QIAseq UPX 3" Targeted RNA Panels use unique molecular index (UMIs) and ultraplex (UPX) technology to enable accurate gene expression using 3" RNA-seq from single cells (1–1000), cell pellets and previously isolated total RNA (1 pg to 10 ng). Use our custom builder to design your custom panel for up to 1000 genes. UPX technology allows up to 384 plates of 384 samples (147,456 total samples) to be multiplexed together at one time. QIAseq UPX 3" Targeted Panels are available for 96 or 384 samples. For processing less than 96 samples at a time, use QIAseq UPX 3" Targeted RNA Panel (96) (cat. no. 333041), which is supplied with a breakable plate enabling processing of as few as 24 samples at one time for sequencing. The QIAseq UPX 3" Targeted RNA Panel (96-M) (cat. no. 333042) is for processing 96 samples in parallel 4 times (384 samples). The QIAseq UPX 3" Targeted RNA Panel (384) (cat. no. 333043) is for processing 384 samples simultaneously. QIAseq UPX 3" Targeted RNA Panels are only compatible with QIAseq UPX Targeted RNA Indices, which must be used during library construction for correct indexing.
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- Product Details
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Cat No./ID:333041 QIAseq UPX 3" Targeted RNA Panel (96) Go to GeneGlobeFor 3" targeted RNA sequencing of 96 cells, cell pellets or ultralow-input RNA samples: Kit contains a breakable 96-well plate with lyophilyzed reagents for cell lysis, cDNA synthesis with Unique Molecular Indexing along with a gene-specific panel for 3" RNA-seq library construction; for a total of 96 samples (up to 1000 genes per sample) |
Cat No./ID:333042 QIAseq UPX 3" Targeted RNA Panel (96-M) Go to GeneGlobeFor 3" targeted RNA sequencing of 4 x 96 cells, cell pellets or ultralow-input RNA samples: Kit contains a 96-well plate with reagents for cell lysis, cDNA synthesis with Unique Molecular Indexing along with agene-specific panel for 3" RNA-seq library construction; for a total of 384 samples (up to 1000 genes per sample) |
Cat No./ID:333043 QIAseq UPX 3" Targeted RNA Panel (384) Go to GeneGlobeFor 3" targeted RNA sequencing of 384 cells, cell pellets or ultralow input RNA samples: Kit contains RT reaction-ready 384-well plate with lyophilyzed reagents for cell lysis, cDNA synthesis with Unique Molecular Indexing along with a gene-specific panel for 3" RNA-seq library construction; for a total of 384 samples (up to 1000 genes per sample) |
Cat No./ID:333044 QIAseq UPX 3" Targeted RNA 12 index (48) $723.00 Sample indices for multiplexing up to 12 x 96 or 12 x 384 plates per lane (total of 48 plates can be indexed) |
Cat No./ID:333051 QIAseq UPX 3" Targeted 96 Index A (384) $4,135.00 Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed) |
Cat No./ID:333052 QIAseq UPX 3" Targeted 96 index B (384) $4,135.00 Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed) |
Cat No./ID:333053 QIAseq UPX 3" Targeted 96 index C (384) $4,135.00 Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed) |
Cat No./ID:333054 QIAseq UPX 3" Targeted 96 index D (384) $4,135.00 Sample index for multiplexing up to 96 x 96 or 96 x 384 plates per lane (total of 384 plates can be indexed) |
Product Details
Performance
Strong reproducibility at ultra-low input RNA amounts
Due to its LNA-enhanced chemistry, the QIAseq UPX 3’ Targeted RNA Panel workflow exhibits strong reproducibility at ultra-low input RNA amounts (see figure "QIAseq UPX 3’ Targeted RNA Panel workflow exhibits strong reproducibility at ultra-low input RNA amounts").Strong linearity from 10 pg to 1 ng
The QIAseq UPX 3’ Targeted RNA Panel workflow shows strong linearity from 10 pg to 1 ng (see figure "QIAseq UPX 3’ Targeted RNA Panel workflow exhibits strong linearity from 10 pg to 1 ng of total RNA").3" RNA-seq from single cells
QIAseq UPX 3" Targeted RNA Panels enable accurate gene expression using 3" RNA-seq from single cells (see figure "Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System"). The kit also enables sucessful identification of cell subtype clusters (see figure "Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cell Subtype Clusters").Principle
QIAseq UPX 3" Targeted RNA Panels enable high-throughput next-generation sequencing (NGS) of up to 1000 polyadenylated RNAs on Illumina sequencers. These panels are intended for library construction and analysis of single cells, cell pellets and ultralow amounts of purified RNA. Single cells or isolated RNA are reverse transcribed and each RNA molecule is given a Unique Molecular Index (UMI) and well-specific IDs are assigned (up to 384 wells; Cell IDs). Following reverse transcription, all cDNAs are combined, enabling simplified library construction steps to be performed in a single tube (see figure "Principle of the QIAseq UPX 3" Targeted RNA Panel Workflow").
Procedure
Innovative, optimized workflow
QIAseq UPX 3" Targeted RNA Panels enable Sample to Insight, high-throughput next-generation sequencing (NGS) of polyadenylated RNAs from single cells on Illumina NGS instruments. In addition, QIAseq UPX is intended for library construction and analysis of cell pellets (up to 100 cells) and purified RNA (10 pg to 1 ng). QIAseq UPX presents an innovative advantage in that, during reverse transcription, each cell is tagged with a unique ID (up to 384 different IDs) and each RNA molecule is tagged with a unique index. Following reverse transcription, all cDNAs are combined, which enables all subsequent library construction steps to be performed in a single tube. This prevents sample mixup, saves substantial time and dramatically reduces library prep costs. Target enrichment technology further enhances the power of QIAseq UPX by enabling users to sequence specific genes of interest, which effectively increases sequencing depth and sample throughput, while minimizing cost. Universal library amplification then assigns up to 384 different sample IDs. Together, the combination of Cell IDs and the Sample IDs enables up to 147,456 targeted libraries to be sequenced together. Ultimately, QIAseq UPX data analysis enables primary mapping, single-cell clustering analysis and differential expression analysis. Collectively, the Sample to Insight workflow of QIAseq UPX defines a new generation of high-throughput NGS technologies.Applications
- High-throughput screening applications (e.g., toxicology research)
- Single-cell analysis
- Differential gene expression analysis
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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