Qiagen/GeneRead Sequencing Q Kits/185251
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商品介绍
GeneRead Sequencing Q Kits
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For preparation of DNA fornext-generation sequencing(NGS) applications using the QIAGEN GeneReader instrument
- For high quality sequencing performance
- Available in kits for multiple flow cell runs
- Color-coded kit components for easy set-up
- Proven performance with the GeneReader NGS System
Next-generation sequencing (NGS) is a driving force for numerous new andexciting applications, including cancer research, stem cell research, metagenomics, population genetics and medical research. The QIAGEN GeneRead Sequencing Q Kits consists of easy-to-use buffers and reagents that only need to be mixed together before loading onto the GeneReader instrument, saving time andpreventing handling errors. Optimized enzyme and buffer compositions ensure high quality sequencing performance, perfect for research applications that requireprecise data insight. Streamlined GeneRead sequencing protocols enable straightforward automation of sequencing-by-synthesis technology on the GeneReader.
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Cat No./ID:185251 GeneRead UMI Advanced Sequencing Q Kit Inquire Contains reagents, three disposable flow cells and wash buffers; Sufficient to perform three single flow cell runs with 150 (150+7) cycles of sequencing on each flow cell. |
Cat No./ID:185905 GeneRead UMI Advanced Sequencing Q Wash Buffers (9) Log in to see pricing GeneRead UMI Advanced Sequencing Q Wash Buffers (9) |
The GeneRead Sequencing Q Kitsare for Research Use Only. Not for use in diagnosticprocedures.
Product Details
Principle
The GeneRead Sequencing Q Kits provide high quality reagents that are easy to mix and install on the GeneReader for a sequencing run.The GeneReaderworkflow includes the following processes: sequencing primer hybridization, flow cell preparation, reagents preparation, flow cell loading and run start (refer to the QIAGEN GeneReader User Manual for additional information). These procedures are described in detail in the GeneReader User Manual. GeneReader sequencing chemistry is briefly described in the Procedure sectionbelow. It consists of a unique terminator-dNTP sequencing-by-synthesis paradigm that ensures highly accurate and cost-effective NGS runs.
Procedure
The QIAGEN GeneReader Platform uses a sequencing-by-synthesis (SBS) approach for deciphering theDNA sequence of specific gene targets. Each cycle of SBS involve 3 major steps: DNA extension,signal detection and cleavage.During the extension step, the DNA polymerase incorporates reversible, terminated fluorescent labeleddeoxyribonucleotide triphosphates (dNTPs) to the DNA template. The reversible terminatorsenable the addition of only one fluorescence-labeled nucleotide at a time to the growing strand ofthe DNA template. This step is followed by a signal detection step carried out by the GeneReaderscanner and imaging system, where signal from each bead is recorded. The cycle is completedwith a cleavage step, in which the fluorescence dyes and the terminators are cleaved from theincorporated nucleotides, allowing the next cycle of incorporation. This technique ensures highlyaccurate and cost-effective NGS.DNA libraries are clonally amplified on beads using the GeneRead QIAcube to become a sequencing template. After hybridization of a sequencing primer the primer-template carrying beads are immobilized via direct bead-glass interaction to produce a high-density array on a GeneReader flow cell. To read out the content of templates on each bead, the array of fragments is first subjected to reagents containing uniquely engineered dNTPs, as described above These bases are incorporated by a modified DNA polymerase to the end of the growing strand of DNA in accordance with the base on the complementary strand. The array is subsequently scanned by a high-resolution digital camera and the fluorescent output of each of the four dye colors at each array position is measured and recorded. Finally, the array is exposed to cleavage chemistry to break off the fluorescent dyes and reversible terminators that will then allow additional bases to be added. This cycle is then repeated on the GeneReader.
Applications
Foruse withtheQIAGEN GeneReader instrument.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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