Qiagen/GeneRead克隆Amp Q试剂盒/185001
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GeneRead Clonal Amp Q Kit
Print
For clonal amplification of DNAlibraries for next-generation sequencing (NGS) applications using the QIAGEN GeneRead QIAcube instrument
- Generation of sequencing-ready amplified libraries
- Multiplex clonal amplification: each library pool can include up to 12 uniquely bar coded DNA library samples
- Automated on the GeneRead QIAcube
- Proven performance with the GeneReader NGS System
Next-generation sequencing (NGS) is a driving force for numerous applications, including cancer research. After the completion of library preparation using the GeneRead DNA Library Q Kit, the GeneRead Clonal Amp Q Kit is used to prepare sequencing templates using clonal amplification.
- Buy Products
- Product Details
Buy Products
Cat No./ID:185001 GeneRead Clonal Amp QKit (4) Inquire Clonal amplification of 4 library pools; Reagents andBuffers for library concentration normalization and pooling, emulsion making, breaking and pooling, and library enrichment |
The GeneRead Clonal Amp Q Kit is for Research Use Only. Not for use in diagnostic procedures.Please note: The GeneReader NGS System is currently only available with proprietary new sequencing chemistry in the US. Legacy sequencing chemistry is only available ex-US.
Product Details
Principle
The GeneRead Clonal Amp Q Kit enables processing of DNA library samples and pooling of multiple DNA library samples that have been uniquely bar coded during the library preparation process. The clonal amplification workflow is comprised of emulsion making, emulsion PCR, emulsion breaking and the enrichment of live beads. At the completion of the entire clonal amplification workflow, the DNA library sample/pools will have been clonally amplified and, after enrichment, are ready for sequencing.
Procedure
The 5-step workflow is outlined below:
Library concentration normalization and pooling
- Manual normalization of the DNA library sample(s) concentration and pooling (if multiplexing is to be performed) are performed.
Emulsion making
- The emulsion PCR beads are denatured in preparation for binding to the DNA library pool to eliminate bead clumping.
- Automated emulsion droplet formation is carried out on the GeneRead QIAcube.
- Upon completion of emulsion making, the PCR plate containing the emulsions is removed from the GeneRead QIAcube workdeck and manually loaded onto a stand-alone thermal cycler for clonal amplification.
Breaking and pooling
- The PCR plate containing the clonally amplified emulsions is loaded back onto the GeneRead QIAcube for the automated processes of pooling, breaking and cleaning of the emulsified library pools.
Enrichment
- Super A Beads are manually washed prior to the enrichment of live beads.
- Automated enrichment of live beads is conducted on the GeneRead QIAcube.
- Enriched beads are recovered and manually transferred from the GeneRead QIAcube into sample tubes.
Manual determination of bead concentration using OD
- Following the clonal amplification workflow, a standard curve is created using Primer Loaded PCR Beads that have not been through the clonal amplification process.
- The optical density of the enriched beads is measured so that the concentration can be extrapolated using the standard curve.
- Enriched beads (clonally amplified and enriched library pools) are then ready for sequencing.
Applications
The GeneRead Clonal Amp Q Kit is designedfor use in the GeneReader NGS System workflow.
Product Resources
Customers who bought these products also bought
- Cat No./ID:181910
GeneRead QIAact Actionable Insights Tumor Panel
Sets of 4 pools containing wet-bench verified primer sets for targeted enrichment of a pathway-focused panel of genes.Select Targets - Cat No./ID:181940
GeneRead DNAseq Panel PCR Kit V2 (12)
GeneRead HotStarTaq polymerase, GeneRead DNAseq Panel 5x PCR Buffer, Nuclease-free Water; enough for 12 samples (4-pool panels) or 24 samples (1-pool panels)$225.00Add To Cart - Cat No./ID:180134
GeneRead DNA FFPE Kit
For 50 preps: QIAamp MinElute Columns, Collection Tubes, Deparaffinization Solution, Uracil-N-Glycosylase, RNase-Free Water, RNase A, and Buffers$328.00Add To Cart - Cat No./ID:185446
GeneRead QIAact Panel Cleanup Kit (48)
Reagents for automated DNA cleanup on the QIAcubeof 48 libraries prepared using the GeneRead QIAact Lung DNA Panel Kit.$80.70Add To Cart - Cat No./ID:929561
QX DNA Size Marker 50–800 bp (50ul) v2.0
DNA size marker with fragments of 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, and 800 bp; concentration 100 ng/ul$83.70Add To Cart - Cat No./ID:929002
QIAxcel DNA High Resolution Kit (1200)
QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips$1,079.00Add To Cart - Cat No./ID:929522
QX Alignment Marker 15 bp/3 kb (1.5 ml)
Alignment marker with 15 bp and 3 kb fragments$97.00Add To Cart - Cat No./ID:990452
Filter-Tips, 1000 µl, wide-bore (1024)
Disposable Filter-Tips, wide-bore, racked; (8 x 128); not required for all protocols. For use with the QIAcube$104.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
