Qiagen/GeneRead尺寸选择套件/用于50个反应：旋转柱和缓冲液/180514
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¥2840.00
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1704.00
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3392242852
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4000-520-616
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商品介绍
GeneRead Size Selection Kit
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For quick and reliable removal of DNA fragments <150 bp forlibrary preparationin NGS applications
- Precise size selection of DNA fragments
- Fast procedure, based on QIAGEN’s proven silica-column technology
- An easy-to-follow protocol, automatable on the QIAcube
The GeneRead Size Selection Kit enables precise size selection and purification of DNA fragments using a fast and convenient spin-column-based procedure. The kit contains MinElute columns and an optimized binding buffer for the selection of DNA fragments larger than 150 bp.Procedurescan be automated on theQIAcube Connect.
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Cat No./ID:180514 GeneRead Size Selection Kit (50) $142.00 For 50 reactions: Spin columns and buffers |
The GeneRead Size Selection Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Fast and precise size selectionThe GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers (see figure Precise size selection). DNA fragments shorter than 150 bp are reliably removed.
Principle
The GeneRead Size Selection Kit combines the convenience of spin-column technology with the selective properties of a uniquely designed binding buffer. MinElute spin columns provided with the kitensure high concentrations of purified DNA for subsequent reactions. The special buffer provided with the kit is optimized for efficient removal of small DNA fragments such as adapters and adapter dimers. Larger DNA fragmentsadsorb to the silica membrane in the presence of optimized concentrations of salt, while contaminants and smaller fragments pass through the column. This leads to a cut-off limit(defined as the length of the shortest fragment that can be visualized by electropherogram) of 150 bp. Impurities suchas enzymes from previous reactions are efficiently washed away, and pure and size-selected DNA is eluted. For optimal adapter monomer and dimer removal, together with high DNA recovery, two subsequent purification steps areperformed for size selection during library preparations for next-generation sequencing.
Procedure
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers. Automatable on the QIAcube, the easy-to-use protocol saves time by eliminating tedious handling procedures, and ensures there is no risk of carryover of potentially inhibitory ethanol or beads (see figure Precise size selection).
Applications
The GeneRead Size Selection Kit enables quick and reliable removal of DNA fragments shorter than 150 bp for subsequent use in library preparation in NGS.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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