Qiagen/GeneRead DNA L Core试剂盒/用于12种反应：用于末端修复、连接和缺口修复的缓冲液和试剂，用于Ion Torrent；文书/180462
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商品介绍
GeneRead DNA L Core Kit
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For fast and efficient preparation of DNA libraries for use in NGS applications
- Fast, one-tube procedure that allows up to 50% time savings
- High yields from minimal amounts of starting material
- Unbiased amplification with an optional, high-fidelity amplification step
- Multiplexing, high-throughput capability
GeneRead Library Prep Kits provide an optimized and efficient workflow for library preparation in next-generation sequencing (NGS) applications. The kits allow fast and efficient preparation of DNA libraries that are for use on NGS platforms from Illumina and the Ion Torrent instrument from Life Technologies.
GeneRead Library Prep Kits can be automated on theQIAcube Connect.
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Cat No./ID:180462 GeneRead DNA Library L Core Kit (12) $309.00 For 12 reactions: Buffers and reagents for end-repair, ligation and nick-repair, for use with Ion Torrentinstruments |
GeneRead DNA L Core Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
High yields of library DNA with uniform coverage distribution
GeneRead Library Prep Kits provide an efficient and optimized workflow to reproducibly generate high yields of DNA library, with minimal sequence bias and low error rates, for use on NGS platforms from Illumina or the Ion Torrent instrument from Life Technologies. Highly efficient ligation reactions and an optional, high-fidelity amplification step ensure superior library yields with uniform coverage distribution, even from as low as 50 ng starting material (see figures High yields of library DNA with uniform coverage distribution and Superior yields of library DNA).Low error rates with minimal sequence bias
To ensure maximum yields from minimum amounts of starting material, GeneRead Library Prep Kits include an innovative high-fidelity master mix that includes GeneRead HiFi Polymerasefor an optional amplification step. GeneRead HiFi Polymeraseisa unique, highly specific and processive enzyme thatdelivers accurate amplification of library DNA with low error rates and minimum bias (see figure Low error rates with minimal sequence bias due to high-fidelity amplification).Lower sequence bias when amplifying GC- and AT-rich regions
In standard PCR amplification procedures, regions of DNA with high AT or GC content can result in little or no amplification, leading to misleading sequence data and NGS results. The GeneRead HiFi Polymerase, together with its unique buffer formulation, ensures uniform amplification of genomic regions that contain highly variable GC content, thereby ensuring even coverage in subsequent sequencing reactions (see figure Better sequence coverage).Principle
GeneRead Library Prep Kits use an optimized, one-tube workflow to reproducibly generate high yields of DNA library for use on NGS platforms from Illumina or the Ion Torrent instrument from Life Technologies. The fast, one-tube procedure uses fewer cleanup steps than library preparation workflows fromother suppliers and includesan optional, high-fidelity library amplificationstep. GeneRead Library Prep Kitssave time and effort while minimizingthe variability caused by handing, as well asthe risk of contamination (see figuresOptimized workflow for Illumina-compatible DNA libraries and Optimized workflow for Ion Torrent-compatible DNA libraries).
Procedure
Procedure for generating Illumina-compatible DNA libraries
Samples consisting of longer DNA fragments are firstsheared into a random library of fragments that are a median fragment size of 300 bp (when using theIllumina MiSeq instrument, version 1), or 500 bp (when using the Illumina MiSeq instrument, version 2), in length. Following fragmentation, the ends of the DNA fragments are repaired and an A-overhang is added at the 3"-end of each strand. Afterwards, adaptors, which are necessary for amplification and sequencing, are ligated to both ends of the DNA fragments. Barcode adapters, which contain a unique identifying sequence, are also available with the GeneRead Library Prep (I) Kit and enable multiplex sequencing reactions to be performed. Fragments are then size selected and purified. Cleanup and size selection can be done using the GeneRead Size Selection Kit (cat. no. 180514), which allows precise, column-based size selection of DNA (see figure Precise size selection). To ensure maximum yields from minimum amounts of starting material, an optional high-fidelity amplification step can also be performed.Procedure for generating Ion Torrent-compatible DNA libraries
Samples consisting of longer DNA fragments are firstsheared into a random library of fragments that are a median fragment size of 400 bp (when using the Ion Torrent PGM instrument), or 200 bp (when using the Ion Proton instrument), in length. Following fragmentation, the ends of the DNA fragments are repaired. Afterwards, adaptors, which are necessary for amplification and sequencing, are ligated to both ends of the DNA fragments. Barcode adapters, which contain a unique identifying sequence, are also available with the GeneRead Library Prep (L) Kit and enable multiplex sequencing reactions to be performed. Fragments are then size selected and purified. To ensure maximum yields from minimum amounts of starting material, an optional high-fidelity amplification step can be performed.Applications
GeneRead Library Prep Kits allow fast and efficient preparation of DNA libraries that are optimized for use on NGS platforms from Illumina and the Ion Torrent instrument from Life Technologies.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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