QIAseq miRNA Library Kit
Gel-free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next-generation sequencing
- Gel-free miRNA sequencing library prep from as little as 1 ng of total RNA
- Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data
- Bead based method to remove adapter dimers and unwanted RNA species
- Integrated Unique Molecular Indices (UMIs) enable quantification of individual miRNA molecules
- Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Center
Optimized reaction chemistry enables robust, miRNA-specific libraries while minimizing reaction biases and eliminating adapter dimers. Unique Molecular Indices (UMIs) tag each miRNA at an early stage, eliminating PCR and sequencing bias. Free, integrated data analysis is available through GeneGlobe for both primary miRNA mapping and UMI analysis as well as secondary differential expression analysis. Want to try this solution for the first time? Request a quote for a trial kit.
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Cat No./ID:331502 QIAseq miRNALibrary Kit (12) $1,187.00 For 12 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers |
Cat No./ID:331505 QIAseq miRNALibrary Kit (96) $7,014.00 For 96 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers |
Cat No./ID:331565 QIAseq miRNA NGS 96 Index IL (96) $1,113.00 Sequencing adapters, primers and indices compatible with Illumina platforms; 96 indices for 96 samples (pre-plated in 96-well plate for ease of use) |
Cat No./ID:331582 QIAseq miRNA NGS 12 Index TF (12) $593.00 Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; 12 indices for 12 samples |
Cat No./ID:331585 QIAseq miRNA NGS 48 Index TF (96) $1,113.00 Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; two 48 indices for 96 samples |
Cat No./ID:331592 QIAseq miRNA NGS 12 Index IL (12) $593.00 Sequencing adapters, primers and indexes compatible with Illumina platforms; 12 indexes for 12 samples |
Cat No./ID:331595 QIAseq miRNA NGS 48 Index IL (96) $1,113.00 Sequencing adapters, primers and indexes compatible with Illumina platforms; two 48 indexes for 96 samples |
Product Details
Mature miRNAs are naturally occurring, 22-nucleotide, non-coding RNAs that mediate posttranscriptional gene regulation. Alterations in miRNA can be correlated with gene expression changes in development, differentiation, signal transduction, infection, aging and disease. Continually growing evidence associates circulating miRNA expression with both normal and disease biology as miRNAs expressed in virtually all biofluids, including serum, plasma, cerebrospinal fluid (CSF) and urine. Specifically with cancers, numerous studies and reviews have associated the presence of various miRNAs with cancer cell proliferation, resistance to apoptosis, invasiveness and differentiation.
Quantification of miRNA expression can be performed using a variety of technologies including next-generation sequencing (NGS) and real-time PCR (qPCR). While NGS is the default tool for novel miRNA discovery, commercially available library preparation kits are tedious and introduce biases. As a result, qPCR has been the “go to” technology for quantification of miRNA expression, until now. QIAseq miRNA definesa new generationin small RNA sequencing products and includes several distinct features not found in other sequencing kits. With the QIAseq miRNALibrary Kit, the power of NGS has been combined with single molecule quantification fromUMIs to produce the most representative expression data possible.Biofluids (such as serum, plasma, CSF and urine), cells, fresh/frozen tissue and FFPE tissues containing total and miRNA are compatible with the QIAseq miRNA Library Kit. Use the QIAseq miRNA to characterize the next circulating miRNA biomarker. Uncover miRNA signatures locked away in FFPE tissues. Completely characterize a new species. The QIAseq miRNA Library Kit has been designed to enhance yields from biofluids such as serum. In the figure Detection of miRNA, the QIAseq miRNA Library Kit shows robust detection of miRNA from serum samples. Mapped reads were then compared to adapter dimers in serum samples. QIAseq miRNA still shows superior mapping of miRNAs even with limited samples (see figure Read distribution in serum samples). With the QIAseq miRNA Library Kit, determine the differential expression of any known or novel miRNAs from any total RNA sample derived from any species.
QIAseq miRNA is the ultimate tool to enhance discovery and expression from large-scale projects with hundreds of samples down to the small pilot focused on a group of target miRNA. QIAseq miRNA offers an unrivaled Sample to Insight solution for differential expression analysis and discovery of novel miRNAs using next-generation sequencing.
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