Qiagen/GeneRead DNA QuantiMIZE Kits/180642
市场价：
¥4620.00
美元价：
2772.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GeneRead DNA QuantiMIZE Kits
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For quantification and qualification of amplifiable DNA prior to NGS
- Provides 2 qPCR assays dispensed onto arrays or in individual tubes
- Quantifies and qualifies DNA that is amplifiable by PCR
- Guides optimization of targeted enrichment conditions
- Requires no serial dilutions
The GeneRead DNA QuantiMIZE System is a qPCR-based approach to determine quantity and quality of DNA that is amenable to amplification by targeted enrichment prior to NGS. The system has been developed for discovery, translational, and clinical researchers who need a cost-effective way to determine the optimum conditions for targeted enrichment of DNA isolated from biological samples, including FFPE. In contrast to other approaches, the system improves the likelihood of a successful NGS run by not only quantifying amplifiable DNA, but also optimizing the target enrichment conditions.Please note: GeneRead DNA QuantiMIZE Array Kit(product number 180642) and GeneRead DNA QuantiMIZE Assay Kit(product number 180654) will be phased out by the end of 2016. Please use QIAseq DNA QuantiMIZE Array Kit(product number 333404) and QIAseq DNA QuantiMIZE Assay Kit(product number333414)instead.
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Cat No./ID:180642 GeneRead DNA QuantiMIZE Array Kit Go to GeneGlobe2 arrays in Formats A, C, D, or F; or 2 discs in Format R; or 1 array in Format E or G; QuantiMIZE Control gDNA (50µl; GeneRead qPCR SYBR Green Mastermix (2 x 1.35 ml) |
Cat No./ID:180654 GeneRead DNA QuantiMIZE Assay Kit $231.00 Assay 100 and Assay 200 for 400 x 25µl reactions (400µl) each; QuantiMIZE Control gDNA (50µl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4) |
GeneRead DNA QuantiMIZE Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Quantification and qualification of amplifiable DNA by the GeneRead DNA QuantiMIZE System rescues low-quality FFPE samples for NGS analysis. Use of the GeneRead DNA QuantiMIZE System resulted in high-quality libraries that were succesfully sequenced on a MiSeq (see Successful quantification and qualification of DNA by the GeneRead DNA QuantiMIZE system).
Principle
GeneRead DNA QuantiMIZE System utilizes 2 qPCR assays that interrogate 20 genomic loci to determine the amounts of amplifiable DNA in biological samples. The assays, Assay 100 and Assay 200, generate 100 and 200 bp amplicons, respectively. QuantiMIZE Kits use the CT values of Assay 100 and Assay 200 for both the Control samples and the Case samples to quantify and qualify DNA (see Principle of the GeneRead DNA QuantiMIZE System).
Procedure
Genomic DNA extracted from biological samples is mixed with the supplied mastermix. This mixture is then aliquoted onto arrays, if the array kits are used, or mixed with Assay 100 and Assay 200 before being aliquoted onto arrays, if the assay kits are used. PCR is performed and the raw CT values are exported to a web-based data analysis module, which will quantify and qualify DNA as well as provide guidance on DNA amounts and PCR cycles needed to optimize targeted enrichment conditions.
Applications
GeneRead DNA QuantiMIZE Kits can be used to quantify and qualify any DNA sample. They are especially beneficial for low‑quality DNA samples, such as FFPE (see Robust performance with FFPE samples). Additionally, the kits can be used to optimize the conditions needed for successful targeted enrichment of genomic regions from any DNA sample, including low-quantity DNA samples, such as FFPE, and low-quantity DNA samples, such as fine-needle aspirates.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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