Qiagen/QIAseq靶向RNA索引/包含样本索引的试剂盒，足以为总共48个样本编制索引，用于在Illumina平台（试管格式）上为靶向RNA测序编制多达12个样本的索引，以及生成的RNA文库测序所需的引物
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5172.00
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商品介绍
QIAseq Targeted RNA Indexes
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For indexing samples for targeted RNA sequencing and primers necessary for sequencing RNA libraries generated by the QIAseq Targeted RNA Panels
- Sample indexes to index up to 96 samples for Illumina and Ion Torrent sequencers
- Enough of each index for four samples
- Tube format for flexibility and array format for convenience and high-throughput
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Cat No./ID:333114 QIAseq Targeted RNA 12-Index I (48) $431.00 Kit containing sample indexes, enough for a total of 48 samples, for indexing up to 12 samples for targeted RNA sequencing on Illumina platforms (tube format), and primers necessary for sequencing RNA libraries generated by the QIAseq Targeted RNA Panels on Illumina platforms |
Cat No./ID:333117 QIAseq Targeted RNA 96-Index I (384) $1,296.00 Kit containing sample indexes, enough for a total of 384 samples, for indexing up to 96 samples for targeted RNA sequencing on Illumina platforms (tube format), and primers necessary for sequencing RNA libraries generated by the QIAseq Targeted RNA Panels on Illumina platforms |
Cat No./ID:333127 QIAseq Targeted RNA 96-Index HT l (384) $1,296.00 Kit containing sample indexes, enough for a total of 384 samples, for indexing up to 96 samples for targeted RNA sequencing on Illumina platforms (array format), and primers necessary for sequencing RNA libraries generated by the QIAseq targeted RNA panels on Illumina platforms |
Cat No./ID:333214 QIAseq Targeted RNA 12-Index L (48) $431.00 Kit containing sample indexes, enough for a total of 48 samples, for indexing up to 12 samples for targeted RNA sequencing on Ion Torrent platforms (tube format) |
Cat No./ID:333217 QIAseq Targeted RNA 96-Index HT L (384) $1,296.00 Kit containing sample indexes, enough for a total of 384 samples, for indexing up to 96 samples for targeted RNA sequencing on Ion Torrent platforms (array format) |
QIAseq Targeted RNA Indexes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
- Accuracy: Innovative digital sequencing (molecular barcode counting) eliminates PCR duplication and amplification bias to deliver the most accurate results (see figure Unbiased and accurate gene quantification) .
- Specificity: The unique combination ofour proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results (see figure Proprietary primer design delivers gene-specific amplicons – 97% specificity).
- Uniformity: The QIAseq Targeted RNA Panel workflow has been optimized to deliver highly uniform sequencing results, to ensure sequencing capacity is utilized very efficiently. In fact, digital sequencing (molecular barcodes) entirely remove this variation in RNAseq counting (see figure Unmatched uniformity – 97% of assays are within 20% of median molecular tag counts).
- Reproducibility: The QIASeq Targeted RNA Panel system demonstrates strong correlations across technical replicates, product lots and instruments with correlation coefficients averaging above >0.99, to ensure reliable detection of differences in expression between biological samples
- Sensitivity: Digital RNA sequencing system is optimized to deliver highly reliable quantification down to ~100 copies of an RNA target in 25 ng total RNA (see figure Positive results with as little as 0.2 copies of RNA per cell).
- Flexibility: QIAseq panels combine the power of NGS with the accuracy of qPCR to allow multiplexing of several samples per NGS while delivering cost-effective results (see figure Simple procedure).
Principle
- Traditional RNA sequencing methods suffer from PCR duplication and amplification bias, resulting in inaccurate gene expression analysis. By introducing molecular barcodes before any amplification takes place, QIAseq Targeted RNA Panels are able to eliminate this issue to deliveraccurate and digital quantification of genes (see figure Unbiased and accurate gene quantification).
- A unique feature of the QIAseq Targeted RNA Panels is the set of built-in control assays. The gDNA assays control for any gDNA contamination in the RNA sample to ensure reproducible results. The housekeeping gene (HKG) assays are used to normalize data, thereby making sample-to-sample and run-to-run comparisons possible.
Procedure
- The QIAseq Targeted RNA Panels workflow begins with converting total RNA into cDNA (see figure Simple procedure). The workflow requires minimal RNA input: as little as 25 ng total RNA can be used. No enrichment or depletion steps are necessary. The molecular barcoding step makes use of molecularly barcoded gene-specific primer (GSP1) in a multiplex primer panel (targeting 12-1000 genes) and an input of 20ng of cDNA equivalent (cDNA made from 20 ng of total RNA). After the barcoding step, the uniquely tagged cDNA is purified over beads to remove residual primers, and a PCR is set up with a second pool of gene-specific adapter primers (GSP2) and the RS2 primer, which primes off of a common tag on the GSP1 primers. This reaction insures that intended targets are enriched sufficiently to be represented in the final library. The number of cycles is kept to a minimum to keep PCR-induced variations in amplification to a low level (any variations are easily corrected and accounted for with the molecular barcodes). Another quick cleanup with beads is performed, and a universal PCR is run with RS2 and FS2 primers, which also adds sample-indexing barcodes to each sample. A final cleanup with beads is performed and the library is complete, and ready for quantification and sequencing.
- An integral component of the QIAseq Targeted RNA Panels is data analysis and insight. Data analysis modules have been developed that are comprehensive, yet easy to use. Using these modules require no bioinformatics expertise. Starting with raw reads directly off the sequencer, the QIAseq targeted RNA data analysis tools at QIAGEN’s GeneGlobe portal, provide you with gene counts and fold changes, as well as links for pathway analysis.
Applications
- Biomarker research
- Confirmation of whole transcriptome sequencing data
- Confirmation of microarray data
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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