Qiagen/GeneRead DNAseq Custom Panel V2/181902
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商品介绍
GeneRead DNAseq Custom Panel V2
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For targeted enrichment of a customized set of genes or genomic regions specific for your NGS needs
- Outstanding sequencing performance
- As little as 10 ng DNA needed
- Compatible with many samples types, including FFPE samples
- Can be used on any sequencing platform
- Customizable for any region in the genome
GeneRead DNAseq Custom Panels V2 are the simplest tools for analyzing the genetic variants of a customizedpanel of genes or genomic regions via next-generation sequencing. Each panel consists of multiplex PCR primer sets, sufficient for 480 samples, to amplify genomic regions of interest tailored to your specific NGS needs. Our primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.
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Cat No./ID:181902 GeneRead Custom DNAseq Gene Panels Inquire Primer sets for targeted enrichment of genomic regions of interest |
GeneRead DNAseq Custom Panel V2 are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The performance of GeneRead DNAseq Custom Panels V2 is assessed by three criteria: design coverage, specificity, and coverage uniformity. Greater than 90% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 90% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.
Principle
The GeneRead DNAseq Custom Panels V2 employ overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see figureMultiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into 4 pools, thereby increasing the specificity of coverage without primer dimers and nonspecific amplifications. Following amplification and purification, enriched regions from each sample are pooled together, yieldingone library preparation for each sample.Virtual panels are custom panels created from gene lists compiled by bioinformatics experts for specific diseases not covered by the GeneRead DNAseq Targeted Panels V2 catalog. For a complete list, scroll down to Applications.
Procedure
GeneRead DNAseq Custom Panels V2 are part of a total workflow for targeted next-generation sequencing (seeGeneRead DNAseq Targeted Panels V2 workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or the GeneRead DNA FFPE Kit are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE System, and then use the GeneRead DNAseq Custom Panels V2 in combination with GeneRead DNAseq Panel PCR Kit V2 to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library with the GeneRead Library Prep Kits and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Perform NGS and analyze your data using the QIAGEN NGS Data Analysis Web Portal.Note: The GeneRead DNAseq Custom Panel Builder is under construction. To build your custom panel, please email your design request to BRC.Custom@qiagen.com, and one of our scientists will follow up with you.
Applications
GeneRead DNAseq Custom Panels V2 are highly suited for enriching a panel of any genomic regions of interest in the human genome to determine genetic variants through NGS analysis. Virtual panels are highly suited for enriching a panel of recommended genomic regions of interest for particular diseases and pathways.
| Panel name | Gene list | # amplicons | Catalog number |
| Autism | iADSL, AFF2, AP1S2, ARX, ATRX, BCKDK, BRAF, CACNA1C, CASK, CDKL5, CHD7, CNTNAP2, CREBBP, DHCR7, DMD, EHMT1, FGD1, FMR1, FOLR1, FOXG1, FOXP1, FOXP2, GABRB3, HPRT1, KDM5C, L1CAM, MBD5, MECP2, MED12, MEF2C, MET, MID1, NHS, NIPBL, NLGN3, NLGN4X, NR1I3, NRXN1, NSD1, OPHN1, PAFAH1B1, PCDH19, PHF6, PNKP, PQBP1, PTCHD1, PTEN, PTPN11, RAB39B, RAI1, RELN, SCN1A, SLC2A1, SLC9A6, SMARCB1, SMC1A, TCF4, UBE2A, UBE3A, VPS13B, ZEB2 | 5580 | CNGHS-00425Z-2806,CNGHS-00426Z-2774 |
| BRCA1 & BRCA2 | BRCA1, BRCA2 | 276 | MNGHS-00363Z-276 (24 samples) & CNGHS-00432Z-276 (500 samples) |
| EGFR Pathway | EGFR, AKT1, BRAF, KRAS, HRAS, NRAS, MAP2K1, PIK3CA, PTEN | 499 | CNGHS-00430Z-499 |
| Epilepsy | ABAT, ABCB1, ADSL, ALDH7A1, ARFGEF2, ARHGEF9, ARX, ASPM, ATP1A2, ATP6AP2, ATR, CACNA1A, CACNA1H, CACNB4, CASK, CASR, CCL2, CDK5RAP2, CDKL5, CDON, CENPJ, CEP152, CHRNA2, CHRNA4, CHRNB2, CLCN2, CLN3, CLN5, CLN6, CLN8, CNTNAP2, CSTB, CTSD, DCX, EFHC1, EFHC2, EMX2, EPM2A, FLNA, FLVCR2, FOLR1, FOXG1, FOXH1, GABRA1, GABRB3, GABRD, GABRG2, GAMT, GATM, GLI2, GPR56, GRIN1, GRIN2A, HCN1, HCN4, KCNA1, KCNJ10, KCNJ11, KCNMA1, KCNQ2, KCNQ3, KCNV2, LGI1, MAGI2, MBD5, MCPH1, MECP2, MEF2C, MFSD8, MTHFR, NDE1, NDUFA1, NHLRC1, NODAL, NRXN1, OPHN1, OPRM1, PAFAH1B1, PCDH19, PCNT, PHF6, PLCB1, PNKP, PNPO, POLG, PPT1, PRICKLE1, PRICKLE2, PRRT2, PTCH1, RELN, SCN1A, SCN1B, SCN2B, SCN3A, SCN3B, SCN4A, SCN4B, SCN5A, SCN8A, SCN9A, SHH, SIX3, SLC25A19, SLC25A22, SLC2A1, SLC9A6, SPTAN1, SRPX2, STIL, STXBP1, SYN1, TCF4, TGIF1, TPP1, TSEN2, TSEN34, TSEN54, UBE3A, VANGL1, WDR62, ZEB2, ZIC2 | 8960 | CNGHS-00422Z-2950 & CNGHS-00423Z-3114 & CNGHS-00424Z-2896 |
| Mitochondria | ATP6, ATP8, COX1, COX2, COX3, CYTB, ND1, ND2, ND3, ND4, ND4L, ND5, ND6, MT | 346 | CNGHS-00442Z-346 |
| MyelodysplasticSyndromes | ASXL1, CBL, DNMT3A, EZH2, IDH1, IDH2, NRAS, RUNX1, TET2, TP53, SF3B1, SRSF2 | 960 | CNGHS-00431Z-960 |
| RTK Signaling | ABL1, AKT1, ALK, BRAF, CBL, CRLF2, CSF1R, CTNNB1, EGFR, ERBB2, FBXW7, FGFR2, FGFR3, FLT3, GNAQ, GNAS, HRAS, JAK2, KIT, KRAS, MAP2K1, MET, NOTCH1, NOTCH2 | 1816 | CNGHS-00429Z-1816 |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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