Qiagen/定制LNA寡核苷酸/339400
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商品介绍
Custom LNA Oligonucleotides
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For experiments requiring custom-designed, LNA-enhanced oligonucleotides
- Customizeyour own LNA oligonucleotides
- Use your own design or let QIAGEN"s LNA experts design them for you
- Select from a wide range of labels and modifications
- Benefit from the easy-to-use online design tool
Custom LNA Oligonucleotides are ideal for studies involving short or very similar sequences. The high affinity of an LNA-enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity, when compared with traditional DNA or RNA oligos. In many cases, LNA-enhanced oligonucleotides can be used to distinguish between sequences differing by only a single nucleotide, a feature that can be critical for the success of many experiments. Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!For Custom LNA Oligonucleotide Large Scale and Custom LNA Oligonucleotide Manual Design, pleasecontact us for ordering and for further information, or email ourSupport Team directly.
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- Product Details
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Cat No./ID:339400 Custom LNA Oligonucleotide (25 nmol synthesis) Go to GeneGlobe25 nmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Cat No./ID:339405 Custom LNA Oligonucleotide Manual Design (25 nmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 25 nmol |
Cat No./ID:339406 Custom LNA Oligonucleotide Manual Design (100 nmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 100 nmol |
Cat No./ID:339407 Custom LNA Oligonucleotide Manual Design (250 nmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 250 nmol |
Cat No./ID:339408 Custom LNA Oligonucleotide Manual Design (1 µmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 1 µmol |
Cat No./ID:339409 Custom LNA Oligonucleotide Manual Design (5 µmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 5 µmol |
Cat No./ID:339410 Custom LNA Oligonucleotide Manual Design (10 µmol synthesis) Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 10 µmol |
Cat No./ID:339411 Custom LNA Oligonucleotide (100 nmol synthesis) Go to GeneGlobe100 nmol synthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Cat No./ID:339412 Custom LNA Oligonucleotide (250 nmol synthesis) Go to GeneGlobe250 nmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Cat No./ID:339413 Custom LNA Oligonucleotide (1 µmol synthesis) Go to GeneGlobe1 µmol synthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Cat No./ID:339414 Custom LNA Oligonucleotide (5 µmol synthesis) Go to GeneGlobe5 µmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Cat No./ID:339415 Custom LNA Oligonucleotide Large Scale (A) Go to GeneGlobeCustom LNA Oligonucleotide Large Scale; various purification and labeling options; available in amounts between 5 mg – 1 kg |
Cat No./ID:339416 Custom LNA Oligonucleotide Large Scale (B) Go to GeneGlobeCustom LNA Oligonucleotide Large Scale; various purification and labeling options; available in amounts between 5 mg – 1 kg |
Cat No./ID:339417 Custom LNA Oligonucleotide (10 µmol synthesis) Go to GeneGlobe10 µmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options available |
Custom LNA Oligonucleotides are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
LNA oligonucleotides exhibit unprecedented thermal stability when hybridized to a complementary DNA or RNA strand. For each incorporated LNA monomer, the melting temperature (Tm) of the duplex increases by 2–8°C (see figure Replace DNA with LNA for higher melting temperature). In addition, LNA oligonucleotides can be made shorter than traditional DNA or RNA oligonucleotides and still retain a high Tm. This is important when the oligonucleotide is used to detect small or highly similar targets.Since LNA oligonucleotides typically consist of a mixture of LNA and DNA or RNA, it is possible to optimize the sensitivity and specificity by varying the LNA content of the oligonucleotide. Incorporation of LNA into oligonucleotides has been shown to improve sensitivity and specificity for many hybridization-based technologies including PCR, microarrays and in situ hybridization (ISH).Tm normalization enables robust detection, regardless of GC content. The Tm of a nucleotide duplex can be controlled by varying the LNA content. This feature can be used to normalize the Tm across a population of short sequences with varying GC content. For AT-rich nucleotides, which give low melting temperatures, more LNA is incorporated into the LNA oligonucleotide to raise the Tm of the duplex. This enables the design of LNA oligonucleotides with a narrow Tm range, which is beneficial in many research applications such as microarrays, PCR and other applications in which sensitive and specific binding to many different targets must occur under the same conditions simultaneously. The power of Tm normalization is demonstrated by the comparison of DNA and LNA probes for detection of miRNA targets with a range of CG content (see figure The power of Tm normalization).
Principle
Use the guidelines below when designing your own Custom LNA Oligonucleotides:
- LNA will bind very tightly to other LNA residues. Avoid self-complementarity and cross-hybridization to other LNA-containing oligonucleotides
- Keep the GC content between 30–60%
- Avoid stretches of more than 4 LNA bases, except when very short (9–10 nucleotides) oligonucleotides are designed
- Avoid stretches of 3 or more Gs or Cs
- For novel applications, design guidelines may have to be established empirically
Applications
LNA oligonucleotides can be successfully used in a wide range of applications, including:
- miRNA research
- Small RNA research
- SNP genotyping
- mRNA antisense oligonucleotides
- Allele-specific PCR
- RNAi
- DNAzymes
- Fluorescence polarization probes
- Molecular beacons
- Microarray gene expression profiling
- Gene repair/exon skipping
- Splice variant detection
- Comparative genome hybridization (CGH)
Product Resources
Product citations in scientific articles
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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