Qiagen/定制LNA mRNA检测探针/339500
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商品介绍
Custom LNA mRNA Detection Probes
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For ultra-sensitive detection of mRNA and lncRNA by in situ hybridization and Northern blotting
- Sensitivity and specificity superior to DNA probes and riboprobes
- Short probes ideal for discriminating highly similar sequences such as splice variants and isoforms
- Online tools provide optimally designed LNA-enhanced probe in minutes
- Available with a wide selection of labels
- No cloning expertise required
Custom design your own LNA-enhanced detection probes for any mRNA or lncRNA target in just minutes using our advanced online design tools. Custom LNA mRNA Detection Probes allow you to easily detect short RNAs and discriminate between highly similar RNAs. High affinity for their target sequences allows use of stringent hybridization and washing conditions, despite short probe lengths.
Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!
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Cat No./ID:339500 Custom LNA mRNA Detection Probes (250 nmol synthesis) Go to GeneGlobe250 nmol synthesis Custom LNA mRNA Detection Probes with option of different labels, provided in tube format |
Cat No./ID:339501 Custom LNA mRNA Detection Probes (1 µmol synthesis) Go to GeneGlobe1 µmol synthesis Custom LNA mRNA Detection Probes with option of different labels, provided in tube format |
Cat No./ID:339508 Control for Custom LNA mRNA Detection Probes (5 nmol) Go to GeneGlobe5 nmol Control for Custom LNA mRNA Detection Probes |
Custom LNA mRNA Detection Probes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
mRNAs can be detected using either specific LNA mRNA Detection Probes, or with an LNA-enhanced poly(T) probe, which detects the poly(A) tail of mRNA transcripts (see figures Custom LNA mRNA Detection Probes give higher signal and lower background than DNA probes, Custom LNA mRNA Detection probes produce a nuclear signal from an RNA, undetectable by the DNA probe, and Detection of an mRNA transcription site using a Custom LNA mRNA Detection Probe).Custom LNA mRNA Detection Probes can also be used to discriminate between different splicing variants, isoforms and to detect the trinucleotide repeats associated with some diseases.In addition, the probes aregreat for detecting short non-coding RNAs and have been widely used for miRNA detection. In some cases, LNA Detection Probes give a signal intensity over 20 times higher than that from DNA probes (see figure Custom LNA mRNA Detection Probes give stronger signals than DNA probes).
Principle
Features
Custom LNA mRNA Detection Probes are great for detecting short RNAs and for discriminating between highly similar RNAs. The high affinity of these probes for their target sequences allows use ofstringent hybridization and washing conditions, despite the short probe lengths. Our online design tool enables you to easily design highly sensitive LNA probes for your specific mRNA sequence.Product description
Custom LNA mRNA Detection Probes are available with a large selection of 3" or 5" labels or inready-to-label format for your own custom labeling. Double (5" and 3") DIG-labeled probes are the most sensitive detection probes available, due tothe synergistic effect between the twolabels. Therefore, we generally recommend this labeling option. The design toolallows you to choose between several different probe synthesis scales and modifiers.Probe design process
Our online design tool uses a sophisticated algorithm to quickly identify optimal LNA-enhanced probes for your target mRNA. The tool quickly evaluates more than 5,000 probe designs based on over 20 design criteria. This process ensures that high-quality probes can be designed for any mRNA sequence. The design criteria include the following:- Optimization of probe length and the number and positions of LNA bases within the probe: ensures that the probes have the correct melting temperatures and avoids any potential self-hybridization.
- A detailed analysis of the probe"s LNA pattern, based onour extensive knowledge of oligonucleotide design rules: ensures that LNA bases are not placed in positions where they could negatively affect the performance of the probe.
- Calculations of the target’s secondary structure:ensures that the probes only target accessible regions of the mRNA.
- BLAST searches:ensure that the probes will only target the relevant mRNA sequence.
Applications
Custom LNA mRNA Detection Probes are great for in situ hybridization applications requiring a short probe, such as in the detection of short sequences or for discriminating between closely related sequences.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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