Qiagen/miRCURY LNA miRNA-ISH优化试剂盒（FFPE）/2x无甲酰胺miRNA-ISH缓冲液，蛋白酶K/339450
市场价：
¥16360.00
美元价：
9816.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRCURY LNA miRNA ISH Optimization Kits (FFPE)
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For setup and optimization of miRNA in situ hybridization (ISH) experiments
- Convenient setup and optimization ofmiRNA in situ hybridization (ISH) experiments
- Superior sensitivity and specificity withdouble DIG-labeled detection probes and optimized reagents
- Highly robust and supports high-throughput and individual miRNA localization studies
- Fast and easy one-day protocol
- High flexibility with no advanced instruments required
- Validated in a wide range of tissues, including FFPE samples
The miRCURY LNA miRNA ISH Optimization Kits (FFPE) offer all the essential reagents to set up and optimize miRNA in situ hybridization (ISH) experiments in the lab. These kits include double DIG-labeled miRCURY LNA miRNA Detection Probes, ISH buffer and reagents.
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- Product Details
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Cat No./ID:339450 miRCURY LNA miRNA ISH Buffer Set (FFPE) $818.00 2x Formamide-free miRNA ISH buffer, Proteinase K |
Cat No./ID:339451 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 1 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 1 (miR-1); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-1p |
Cat No./ID:339452 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 2 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 2 (miR-21); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-21-5p |
Cat No./ID:339453 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 3 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 3 (miR-122); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-122p-5p |
Cat No./ID:339454 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 4 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 4 (miR-124); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-124-3p |
Cat No./ID:339455 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 5 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 5 (miR-126); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-126-3p |
Cat No./ID:339456 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 7 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 7 (miR-145); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-145-5p |
Cat No./ID:339457 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 8 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 8 (miR-205); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-205-5p |
Cat No./ID:339458 miRCURY LNA miRNA ISH Optimization Kit (FFPE) 9 $2,231.00 miRCURY LNA miRNA ISH Optimization Kit 9 (miR-223); includes controls, buffer and proteinase K for 400 slides; detects hsa-miR-223-3p |
Cat No./ID:339459 miRCURY LNA miRNA ISH Buffer and Controls $1,490.00 Scramble miRNA Negative Control Probe, U6 snRNA Positive Control Probe, 2x Formamide-free miRNA ISH buffer, Proteinase K; for 400 slides |
miRCURY LNA miRNA ISH Optimization Kits (FFPE) are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Double DIG-labeled probes for superior sensitivity
The miRCURY LNA miRNA ISH Optimization Kits (FFPE) use double DIG-labeled miRCURY LNA miRNA Detection Probes. These probes offer exceptional sensitivity and specificity, so they even can be used to detect low-abundance miRNAs.All of the kits have been thoroughly validated for use with a wide range of tissues to ensure the highest level of performance (see table below).| Product | Unique probe | Examples of miR-expressing cells | Data figure |
|---|---|---|---|
| Kit 1 | miR-1 | Skeletal muscle cells, myocytes, myocardiocytes | miR-1 detection in adult mouse heart |
| Kit 2 | miR-21 | Cancer-associated fibroblasts, some immune cells | miR-21 detection in colon adenocarcinoma |
| Kit 3 | miR-122 | Hepatocytes | miR-122 detection in mouse liver |
| Kit 4 | miR-124 | Neurons | miR-124 detection in human brain |
| Kit 5 | miR-126 | Endothelial cells | miR-126 detection in colon wall |
| Kit 7 | miR-145 | Smooth muscle, vascular smooth muscle (pericytes), myoepithelial cells | miR-145 detection in human colon |
| Kit 8 | miR-205 | Basal keratinocytes, myoepithelium, SCC cancer cells | miR-205 detection in human breast carcinoma |
| Kit 9 | miR-223 | Myeloid, granulocytic, and monocytic compartments of the hematopoietic system | miR-223 detection in esophagus cancer |
The ideal way to optimize miRNA ISH experiments
All of the miRCURY LNA miRNA ISH Optimization Kits (FFPE) include a double DIG-labeled scrambled probe and a 5’ DIG-labeled U6 control probe. The U6 probe targets U6 snRNA, a small RNA expressed in most tissues, making it an ideal positive control probe in the initial stages of setting up an ISH experiment. The scrambled probe is used as a negative control probe.In addition, a kit-specific, double DIG-labeled miRCURY LNA miRNA Detection Probe is provided with each kit. These probes can be used as positive control probes in the experiment, once the initial optimization has been performed.A solution for many problems
The miRCURY LNA miRNA ISH Optimization Kits (FFPE) are very flexible, offering solutions for many different experimental setups without the need for advanced instruments. Some applicationsinclude:
- Study of cellular and sub-cellular miRNA localization
- Examination of spatial miRNA expression
- miRNA expression analysis during developmental processes
- ISH analysis of FFPE samples
- Biomarker discovery in FFPE samples
- Potential miRNA ISH in other species where the miRNA target sequence for thekit-specific LNA probe is conserved
- Evaluation of results from functional studies
Principle
Features
ThemiRCURY LNA miRNA ISH Optimization Kit (FFPE) is a great tool to help you get started with or to optimize miRNA in situ hybridizations (ISH) on formalin-fixed paraffin embedded (FFPE) tissue samples.Based on the highly successful double (5’ and 3’) DIG-labeled miRCURY LNA miRNA Detection Probes, the kits provide the sensitivity and specificity needed to detect miRNAs (see figurePrinciple of the miRCURY LNA miRNA ISH Optimization Kit). In addition, the reagents included have been specifically adapted for use with LNA-enhanced probes in FFPE tissue sections.Coverage
Eight different miRCURY LNA miRNA ISH Optimization Kits are available. Each kit comes with positive and negative control probes, hybridization buffer and Proteinase K. In addition, a unique, tissue-specific miRCURY LNA miRNA Detection Probe is included in each kit.Each kit includes:- Unique miRNA LNA probe (double DIG-labeled, kit specific)
- Scrambled LNA probe (double DIG-labeled, negative control)
- U6 LNA probe (5’ DIG-labeled, positive control)
- Hybridization buffer (2x, formamide-free)
- Proteinase K
Procedure
The One-Day miRNA ISH Protocol included in the kit handbook has been thoroughly tested and optimized. Our scientists have eliminated several steps normally associated with ISH, such as pre-hybridization, post-fixation and acetylation, making the protocol very robust, easy to optimize and ideal for high-throughput use. Both the hybridization and washing steps of the One-Day miRNA ISH Protocol are performed using completely formamide-free buffers, which makes working with the kits less cumbersome.
Applications
The miRCURY LNA miRNA ISH Optimization Kits (FFPE) can be used for a large number of applications, including cellular and sub-cellular miRNA localization studies and determination of spatial miRNA expression. The robustness of the procedure makes it ideal for use in both high-throughput ISH analysis and localization studies of individual miRNAs.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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