Qiagen/ipsogenRT试剂盒/679913
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商品介绍
ipsogen RT Kit
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For reverse transcription of total RNA in research use only
- High-quality reagents for reverse transcription
- Reliable reagents for reverse transcription
- Accurate and reproducible reverse transcription
The ipsogen RT Kit is intended for research use only and is not for use in diagnostic procedures. The kit provides reagents optimized for reliable and accurate reverse transcription using the Rotor-Gene Q or other thermal cycler instruments. The kit is intended for reverse transcription of total RNA into cDNA for downstream real-time PCR applications.
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Cat No./ID:679913 ipsogen RT Kit (33) Log in to see pricing For 33 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Principle
The ipsogen RT Kit is a ready-to-use kit for reverse transcription of total RNA into cDNA using reverse transcription-PCR (RT-PCR). The kit provides accurate and reproducible reverse transcription of total RNA into cDNA. Reverse transcriptase is a multifunctional enzyme with 3 distinct enzymatic activities: an RNA-dependent DNA polymerase, a hybrid-dependent exoribonuclease (RNase H), and a DNA-dependent DNA polymerase. In vivo, the combination of these 3 activities allows transcription of the single-stranded RNA genome into double-stranded DNA. The RNA-dependent DNA polymerase activity (reverse transcription) transcribes cDNA from an RNA template. This activity allows synthesis of cDNA for cloning, PCR, and sequencing.
Procedure
The first step is extraction and determination of quality and quantity of total RNA. The second step is reverse transcription of total RNA into cDNA using the ipsogen RT Kit. The use of the ipsogen RT Kit allows reliable and accurate reverse transcription of total RNA. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen RT Kit enables reliable and accurate reverse transcription of total RNA for research use only.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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