Qiagen/ipsogen JAK2 MutaScreen Kits/673012
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商品介绍
ipsogen JAK2 MutaScreen Kits
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For qualitative or semi-quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR
- Reliable qualitative or semi-quantitative detection
- Multiplex analysis with no need for optimization
- Easy workflow with ready-to-use solutions
The ipsogen JAK2 MutaScreen Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection, and qualitative or semi-quantitative analysis of the JAK2 V617F/G1849T mutation in genomic DNA.
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Cat No./ID:673012 ipsogen JAK2 MutaScreen Kit (10) Log in to see pricing For 10 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F |
Cat No./ID:673013 ipsogen JAK2 MutaScreen Kit (24) Log in to see pricing For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F |
Cat No./ID:673113 ipsogen JAK2 MutaScreen RS Kit (19) Log in to see pricing For 19 reactions: V617F Positive Control, V617F Negative Control, V617F Reference Scale, Primers and Probes Mix JAK2 wild-type and JAK2 V617F |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
To ensure highest accuracy, ipsogen JAK2 MutaScreen Kits have been optimized to detect the JAK2 V617F/G1849T mutation and use DNA positive and negative controls for qualitative analysis (see figuresRepresentative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA and Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA). ipsogen JAK2 MutaScreen Kits provide a DNA 2% cut-off sample (COS) positivity and a minimum of 25 ng of input sample DNA is recommended.
Principle
ipsogen JAK2 MutaScreen Kits are ready-to-use kits for the detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure JAK2 mutation site identification). The kits provide reliable and reproducible results. ipsogen JAK2 MutaScreen Kits provide a DNA cut-off sample (COS), a DNA positive control (PC), and a negative control (NC) for JAK2 V617F. Use of the cut-off sample and controls enables accurate qualitative analysis of genotyping results. The ipsogen JAK2 MutaScreen RS Kit provides a V617F reference scale.
Procedure
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaScreen Kits provide reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
ipsogen JAK2 MutaScreen Kits enable sensitive and reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation for research use only.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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