Qiagen/ipsogen NPM1 mut A, B&D MutaQuant Kits/677513
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商品介绍
ipsogen NPM1 mut A, B&D MutaQuant Kits
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For quantification of type A, B, and D mutations in NPM1 gene transcripts using real-time PCR
- Sensitive quantitative assay
- Specific quantification of type A, B, and D mutations
- Accurate quantification relative to ABL control gene expression
- Optimal quality control and ISO 13485 certification
- Easy workflow with ready-to-use solutions
ipsogen NPM1 mut A, B&D MutaQuant Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. ipsogen NPM1 mut A, B&D MutaQuant Kits provide reagents optimized for reliable and sensitive detection and quantification of specific mutations (type A, B, and D) in NPM1 gene transcripts.
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Cat No./ID:677513 ipsogen NPM1 mut A MutaQuant Kit (24) Log in to see pricing For 24 reactions: ABL Control Gene Standards, NPM1 MutA Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutA Mutated Gene |
Cat No./ID:677613 ipsogen NPM1 mut B&D MutaQuant Kit (24) Log in to see pricing For 24 reactions: ABL Control Gene Standards, NPM1 MutB Gene Standards, NPM1 MutD Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutB Mutated Gene, Primer and Probe Mix NPM1 mutD Mutated Gene |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
To ensure highest sensitivity, ipsogen NPM1 mut A, B&D MutaQuant Kits have been optimized to detect the type A, B, and D mutations in NPM1 gene transcripts over a wide dynamic range (see figure "Sensitive detection of ABL and NPM1 mut A, B, and D standards").
Principle
ipsogen NPM1 mut A, B&D MutaQuant Kits are ready-to-use kits for the detection and quantification of type A, B, and D mutations in human RNA already identified as carrying the respective mutation in the NPM1 gene (see figure "NPM1 alleles"). Results are quantified relative to expression of the ABL control gene (see figure "ABL control gene transcript"). The kits provide high levels of sensitivity and reproducibility. Each ipsogen NPM1 mut A, B&D MutaQuant Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for each of the NPM1 gene mutations.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of DNA by real-time PCR. Using ipsogen NPM1 mut A, B&D MutaQuant Kits provides reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
ipsogen NPM1 mut A, B&D MutaQuant Kits enable sensitive and reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts for research use only.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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