QIAamp DNA Blood Midi Kit
- Rapid purification of high-quality, ready-to-use DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
QIAamp DNA Blood Midi Kits provide silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Midi Kit is designed for processing 0.3–2 ml fresh or frozen human whole blood. The QIAamp Midi spin columns can be easily processed in a centrifuge or on vacuum manifolds (additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with vacuum manifolds).
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Cat No./ID:51183 QIAamp DNA Blood Midi Kit (20) $233.00 For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) |
Cat No./ID:51185 QIAamp DNA Blood Midi Kit (100) $977.00 For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15ml) |
Product Details
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").
The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery DNA, depending on the starting cell densities (see table).
| Leukocytes per ml | DNA yield (µg) | DNA recovery (%) |
| 2.5 x 105 | 3.0 | 92.3 |
| 1.0 x 106 | 11.0 | 91.7 |
| 5.0 x 106 | 62.4 | 94.5 |
| 1.0 x 107 | 116.3 | 88.1 |
No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.
The QIAamp DNA Blood Midi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Midi Kit processes sample sizes of 0.3–2 ml, with a preparation time of 55 minutes. The typical yield from 1 ml healthy whole blood is 20–60 µg, with an elution volume of 100–400 µl.
QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.
The QIAamp DNA Blood Midi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Midi spin columns with 15 ml collection tubes. Sample sources include:
Specifications
Features | Specifications |
| Applications | PCR, southern blotting |
| Elution volume | 100–400 µl |
| Format | Spin column |
| Main sample type | Whole blood, body fluids |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA, viral DNA |
| Sample amount | 0.3–2 ml |
| Technology | Silica technology |
| Time per run or per prep | 55 minutes |
| Yield | 20–60 µg |
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