Investigator 24plex GO! Kit
- CODIS and European standard markers in one reaction
- Direct amplification technology for reference samples
- Integrated Quality Sensor to support decision making
- PCR speed of around 45 minutes for faster results
- Reduced allelic overlap and unambiguous results
The Investigator 24plex GO! Kit is for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, plus SE33, DYS391, D2S1338, D19S433 and Amelogenin. Amplification is carried out directly from the sample without prior DNA extraction. The kit includes an innovative Quality Sensor, enabling invaluable additional data to be generated for quality control and performance tests. Want to try this solution for the first time? Request a quote for a trial kit.
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Cat No./ID:382426 Investigator 24plex GO! Kit (200) $3,878.00 Primer Mix, Fast Reaction Mix 2.0 including Taq DNA polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO) |
Cat No./ID:382428 Investigator 24plex GO! Kit (1000) $17,983.00 Primer Mix, Fast Reaction Mix 2.0including Taq DNA polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO) |
Cat No./ID:386037 DNA Size Standard 24plex (BTO) $595.00 DNA Size Standard 24plex (BTO) for 800 reactions |
Cat No./ID:386224 Matrix Standard BT6 (50) $175.00 Matrix standard for 6-FAM, BTG, BTY, BTR2, BTP, and BTO, for Applied Biosystems 3500 Genetic Analyzers |
Cat No./ID:386516 Investigator STR GO! LysisBuffer (200) $357.00 Lysis buffer for 200 swab samples |
Cat No./ID:386526 Investigator STR GO!PunchBuffer (200) $340.00 Lysis buffer for 200 samples of epithelial cells on paper |
Cat No./ID:386528 Investigator STR GO!PunchBuffer (1000) $1,727.00 Lysis buffer for 1000 samples of epithelial cells on paper |
Product Details
- If amplification was successful
- If DNA was present in the sample
- If the sample was degraded
- If inhibitors in the sample prevented successful PCR
This additional information will greatly enhance your data analysis andwill helpyou to make crucial decisions with respect to further sample processing. Ultimately, the Quality Sensors will help you reduce unnecessary re-runs, saving you time and money. The optimized design of the two gender markers, Amelogenin and DYS391, enables the Investigator 24plex GO! Kit to have both at a small fragment size, making the gender typing extremely robust. Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.
The STR markers and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator 24plexGO! Kit dyes and corresponding markers".
The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Investigator 24plex GO! Kit technical specifications" for more information.
| Dye | Marker | ||||||
|---|---|---|---|---|---|---|---|
| 6-FAM | Amelogenin | TH01 | D3S1358 | vWA | D21S11 | ||
| BTG | TPOX | DYS391 | D1S1656 | D12S391 | SE33 | ||
| BTY | D10S1248 | D22S1045 | D19S433 | D8S1179 | D2S1338 | ||
| BTR2 | D2S441 | D18S51 | FGA | ||||
| BTP | QS1 | D16S539 | CSF1PO | D13S317 | D5S818 | D7S820 | QS2 |
| Category | Details |
|---|---|
| Volume per amplification reaction | 20 μl |
| Matrix | BT6 |
| Fluorescence labels | 6-FAM, BTG, BTY, BTR2, BTO, BTP |
| Genetic analyzers | Applied Biosystems 3500/3500xL Genetic Analyzers |
| Software for analysis | Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software |
The Investigator 24plex GO! Kit is for direct and reliable generation of DNA profiles from blood on FTA or other paper, buccal cells on FTA or other paper, or buccal swabs. It is highly suited for:
- Criminal database samples
- Paternity testing
- Population genetics studies
- Other human identification and forensics applications
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