Qiagen/Investigator ESSplex SE QS Kit/Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water/381575
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¥43920.00
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26352.00
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3392242852
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4000-520-616
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商品介绍
Investigator ESSplex SE QS Kit
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For multiplex amplification of the European Standard Set (ESS), SE33 and Amelogenin,with an innovative Quality Sensor
- Integrated Quality Sensor for better decision making and data interpretation
- Faster results with a PCR reaction speed of around 60 minutes
- Simple analysis with minimal overlapping allele panels and an extended ladder
- High sensitivity and inhibitor resistance
The Investigator ESSplex SE QS Kit amplifies the full suite of 16 STR markers in the European Standard Set (ESS),including SE33 and the gender-specific Amelogenin, as well as a unique performance control called Quality Sensor (QS). The Quality Sensor provides additional, valuabledata for your sample quality control and PCR performance check. By taking advantage of QIAGEN´s fast-cycling technologies, the kit enablesPCR amplification in just 60 minutes. Highly sensitive, withrapid and reliable results from trace DNA,the Investigator ESSplex SE QS Kitis suitable for all forensic applications and paternity testing.
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Cat No./ID:381575 Investigator ESSplex SE QS Kit (100) $2,196.00 Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water |
Cat No./ID:381577 Investigator ESSplex SE QS Kit (400) $7,934.00 Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water |
The Investigator ESSplex SE QS Kit is intended for molecular biology applications in forensic, human identity and paternity testing. This product is not intended for diagnosis, prevention or treatment of a disease.
Product Details
Performance
The Invesigator ESSplex SE QS Kit utilizes fast-cycling technology to provide reliable results in approximately 60 minutes. The novel Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results. The Investigator ESSplex SE QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
Principle
The Investigator ESSplex SE QS Kit uses QIAGEN"s fast-cycling PCR technology for the simultaneous amplification of 16 STR markers and Amelogenin, as recommended by the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling Group (EDNAP) as the European Standard Set of loci (ESS).The Investigator ESSplex SE QS Kit Primer Mix contains the new innovative performance control Quality Sensor (QS1 and QS2). It provides additional information on sample quality and PCR success. The Quality Sensor, being simultaneously amplified with the STR markers, shows you if you are dealing with:
- Successful PCR amplification
- Failed PCR amplification
- Absence of DNA
- Degraded DNA
- Inhibitors
Procedure
The 16 STR markers, Amelogenin and the Quality Sensorare amplified simultaneously in a single PCR, and labeled with one of the dyes listed in Table 1 "Investigator ESSplex SE QS Kit dyes and corresponding markers".Theloci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as the Applied Biosystems GeneMapper ID-X. See the table "Investigator ESSplex SE QS Kit technical specifications" for more information.
| Dye | Marker | ||||||
| 6-FAM | QS1 | Amelogenin | TH01 | D3S1358 | vWA | D21S11 | QS2 |
| BTG | D163539 | D1S1656 | D19S433 | SE33 | |||
| BTY | D10S1248 | D22S1045 | D12S391 | D8S1179 | D2S1338 | ||
| BTR | D2S441 | D18S51 | FGA |
| Category | Details |
| Volume per PCR | 25 µl |
| Matrix | BT5 |
| Fluorescence labels | 6-FAM, BTG, BTY, BTR, BTO |
| Genetic analyzers | ABI PRISMR 3100/3100-Avant, Applied Biosystems 3130/3130xl orApplied Biosystems 3500/3500xL Genetic Analyzers |
| Software for analysis | Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software |
Applications
The Investigator ESSplex SE QS Kit enables simultaneous amplification of the European Standard Set (ESS), including SE33, and is highly suitable for:
- Criminal database and forensic casework samples
- Paternity testing
- Population genetics studies
- Other human identity testing applications
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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