Qiagen/Investigator Argus X-12 QS Kit/Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water/383223
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¥14180.00
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8508.00
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4000-520-616
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商品介绍
Investigator Argus X-12 QS Kit
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For multiplex amplification of 12 X-chromosomal STRs, D21S11 and Amelogenin, withan innovative Quality Sensor
- Co-amplification of 12 X-chromosomal and one autosomal alignment marker to avoid sample mix-up
- Integrated Quality Sensor for better decision making and data interpretation
- PCR run speed of around 80 minutes for faster results
- Smart primer design with reduced allelic overlap for unambiguous results
- High sensitivity and inhibitor resistance
The Investigator Argus X-12 QS Kit is a multiplex solution for human identification. The 12 X-chromosomal markers, D21S11 and Amelogenin are co-amplified. The autosomal marker, D21S11, enables alignment of the Investigator Argus X-12 QS Kit profile with a profile from any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups. With its innovative Quality Sensor (QS), the Investigator Argus X-12 QS Kit includes a novel internal performance control. This allows you to readily determine if your PCR run was successful or if there was no DNA present in your sample. The kit utilizes QIAGEN"s fast-cycling technology, enabling amplification in just ~80 minutes. Highly sensitive, with rapid and reliable results from trace or reference DNA, the Investigator Argus X-12 QS Kit is suitable for paternity or kinship testing and for gonosomal STR analysis of forensic stains.
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Cat No./ID:383223 Investigator Argus X-12 QS Kit (25) $709.00 Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water |
Cat No./ID:383225 Investigator Argus X-12 QS Kit (100) $2,073.00 Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder Argus X-12 QS, DNA size standard 550 (BTO) and Nuclease-Free Water |
The Investigator Argus X-12 QS Kit is intended for molecular biology applications in forensic, human identity and paternity testing. This product is not intended for diagnosis, prevention or treatment of a disease.
Product Details
Performance
The Investigator Argus X-12 QS Kit utilizes fast-cycling technology to provide reliable results in approximately 80 minutes.The novel Fast Reaction Mix 2.0 is based on proven QIAGEN Multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.The Investigator Argus X-12 QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
Principle
The Investigator Argus X-12 QS Kit uses QIAGEN"s fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping.
The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine:
| Linkage group | |
| 1 (Xp22) | DXS8378 – DS10135 – DXS10148 |
| 2 (Xp11) | DXS7132 – DXS10074 – DXS10079 |
| 3 (Xp26) | HPRTB – DXS10101 – DXS10103 |
| 4 (Xp28) | DXS7423 – DXS10134 – DXS10146 |
- A successful PCR run
- A failed PCR run
- Lack of DNA in your sample
Procedure
The 12 X-chromosomal markers, D21S11 and the gender-specific Amelogenin, are amplified simultaneously in a single PCR run and labeled with one of the dyes listed in the Table 2: "Investigator Argus X-12 QS Kit dyes and corresponding markers".The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications" for more information.
| Dyes | Markers | |||||
| 6-FAM | QS1 | Amelogenin | DXS10103 | DXS8378 | DXS10101 | DXS10134 |
| BTG | DXS10074 | DXS7132 | DXS10135 | |||
| BTY | DXS7423 | DXS10146 | DXS10079 | |||
| BTR | DXSHPRTB | DXS10148 | D21S11 |
| Category | Details |
| Volume per PCR | 25 µl |
| Matrix | BT5 |
| Fluorescence labels | 6-FAM, BTG, BTY, BTR, BTO |
| Genetic analyzers | ABI PRISM 3100/3100-Avant, Applied Biosystems3130/3130xl orApplied Biosystems 3500/3500xL Genetic Analyzers |
| Software for analysis | Applied Biosystems GeneMapper ID,GeneMapper ID-X or Genotyper Software |
Applications
The Investigator Argus X-12 QS Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:
- Kinship and paternity testing, especially deficiency cases
- Population genetics and anthropological studies
- Analysis of stains with female traces on a male background
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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