Qiagen/DNeasymericon96 QIAcube HT试剂盒/用于480个制剂：DNeasy 96板，Qiagen蛋白酶K，缓冲液/69571
市场价：
¥20640.00
美元价：
12384.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DNeasy mericon 96 QIAcube HT Kit
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For automated high-throughput isolation of DNA fromraw or processed food samples
- Purification of DNA from various types of food samples
- No carryover of PCR inhibitors from complex food matrices
- Accelerated CTAB procedure ensures efficient DNA extraction
- Simple and reliable automated processing for cost and time savings
The DNeasy mericon 96 QIAcube HT Kit enables simple, automated purification of DNA from a variety of raw and processed foods on the QIAcube HTsystem. Using proven DNeasy silica-membrane technology in a convenient 96-well format, contaminants and inhibitors from complex food matrices are removed to yield high-quality DNA ready for downstream analysis. The kitforms part of the comprehensive QIAGEN food testing portfolio, which also features assays for pathogen and GMO DNA detection and ingredient authentication.
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- Product Details
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Cat No./ID:69571 DNeasy mericon 96 QIAcube HT Kit (5) $1,032.00 For 480 preps: DNeasy 96 plates, QIAGEN Proteinase K, buffers |
Cat No./ID:950067 QIAcube HT Plasticware $321.00 Plasticware for 480 typical preps on QIAcube HT: 5S-Blocks, 5 Elution Microtubes RS (EMTR), 120x8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad |
The DNeasy mericon Food Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The DNeasy mericon 96 QIAcube HT Kit is designed for rapid, automated purification of DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. DNeasy mericon purification kits provide a universally applicable extraction method that gives reliable results even when using strongly inhibitory, highly processed, fatty, acidic, high-, or low-DNA content foods (see figure High yields of DNA and minimal inhibitor carryover). The QIAcube HT platform, together with the dedicated DNeasy mericon 96 QIAcube HT Kit, enables automated mid- to high-throughput DNA purification from food sample types. Now it is possible to prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure (see figure Purification of DNA from food samples).
Principle
The DNeasy mericon96 QIAcube HT Kit enables automated purification of nucleic acidsfrom up to300 mgfood sample materialusing the QIAcube HT instrument.The procedure yields high-quality DNA that performs well in PCR and other downstream applications. The DNeasy mericon96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | Up to300 mg food material |
| Elutionvolume | 200 μl |
| Duration | 96 samples in approximately73 minutes 24 samples in approximately 42 minutes |
Procedure
The DNeasy mericon 96 QIAcube HT Kit uses a modified cetyltrimethylammonium bromide (CTAB) extraction procedure for rapidautomated purification of DNA from food matrices on the QIAcube HT instrument (see figure Efficient and rapid automated DNA extraction). Homogenized food samples undergo a manual pretreatment step for efficient lysis using Food Lysis Buffer in combination with proteinase K.Chloroform extraction removes any CTAB-protein, CTAB-debris, or CTAB-polysaccharide complexes.Theaqueous phase containing the DNA is transferred to a 96-well S-Block, and all subsequent purification steps are automated on the QIAcube HT instrument.QIAcube HT adds Buffer PB to theaqueous phase and transfers the mixture to the DNeasy 96 plate. As vacuum is applied, nucleic acids are adsorbed onto the silica membrane while contaminants pass through. Washing removes any remaining contaminants and inhibitors, and the DNA is eluted in Buffer EB. The resulting purified DNA is ready for use in a downstream mericon real-time PCR assay.
Applications
The DNeasy mericon 96 QIAcube HT Kit yields high-quality DNA from a variety of raw and processed foodmatrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. Purified DNA is suitable for a range of downstream applications:
- Food safety and quality testing
- Ingredient authentication
- Detection of genetically modified organisms
- Detection of potential allergens
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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