Qiagen/QIAxcel RNA质控试剂盒v2.0/用于100次12个样本：QIAxcel RNA质控凝胶盒、缓冲液、矿物油、QX强度校准标记、QX RNA校准标记、QX RNA大小标记200–6000 nt、QX RNA变性缓冲液、12管条
市场价：
¥24900.00
美元价：
14940.00
联系Q Q：
3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
QIAxcel RNA QC Kit v2.0
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For automated quantitative and qualitative RNA analysis using QIAxcel instruments
- Objective RNA quality measurement with RNA Integrity Score (RIS)
- Robust results using as little as 5 ng/μl of diluted total RNA
- Safety and convenience with ready-to-use gel cartridges
- Higher sensitivityand faster than agarose gel electrophoresis
- Standardized processing with digital data collection
The QIAxcel RNA QC Kit v2.0 provides fully automated quantitative and qualitative analysis of up to 96 RNA samples per run on QIAxcel instruments. Automated sample loading and analysis reduce manual handling of samples, minimizing the risk of RNA degradation and contamination. The system can detect as little as 5 ng/μl of diluted total RNA(10 ng/µl in original RNA eluate) and 50 ng/µl of cRNA or single-stranded cDNA. When used together with the updated QIAxcel ScreenGel software, the new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of eukaryotic RNA samples and allows easy interpretation of sample integrity.The QIAxcel RNA QC Kit v2.0 is only used in combination with theQIAxcel Advanced Systemand the QIAxcel RNA Accessories.
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Cat No./ID:929104 QIAxcel RNA QC Kit v2.0 (1200) $1,245.00 For 100 runs of 12 samples: QIAxcel RNA Quality Control Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, QX RNA Alignment Marker, QX RNA Size Marker 200–6000 nt, QX RNA Denaturation Buffer, 12-Tube Strips |
The QIAxcel RNA QC Kit v2.0 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Fast, sensitive analysis of RNA
RNA quality is a critical factor for reliable results in gene expression studies and miRNA research, in which sensitive technologies such as real-time PCR or microarray analysis are used. Monitoring theintegrity of the initial total RNA sample as well as products generated throughout the entire procedure is crucial, since their integrity strongly influences the predictive power of the detection method. Fully automated QIAxcelinstruments enable fast and sensitive analysis of theintegrity and quantity of total RNA and fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system). Automated sample loading and analysis reduce manual handling of samples, minimizing the risk of RNA degradation and contamination. Thedegradation state of samples can be easily analyzed on QIAxcel instruments (see figures Analysis of degraded RNA and Analysis of plant RNA).Objective quality values with the new RNA Integrity Score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs. RNA analysis on QIAxcel instruments is highly reproducible and gives results comparable to the Agilent Bioanalyzer.Principle
QIAxcelinstruments use capillary gel electrophoresis to enable fast size-based separation of nucleic acids (see figure Sample separation process). Unlike traditional agarose gel electrophoresis, separation is performed in a capillary of a ready-to-use gel cartridge. The samples are automatically loaded into an individual capillary and voltage is applied. The negatively charged nucleic acid molecules migrate through the capillary to the positively charged terminus. As with agarose gel electrophoresis, low-molecular-weight molecules migrate faster than high-molecular-weight molecules. As the molecules migrate through the capillary, they pass a detector which detects and measures the fluorescent signal. A photomultiplier converts the fluorescent signal into electronic data, which are then transferred to the computer for further processing using QIAxcel ScreenGel software. After processing, the data are displayed as an electropherogram or gel image.
Procedure
The QIAxcel RNA QC Kit v2.0 simplifies separation and analysis of RNA, and provides increased user safety. Just a few simple steps are required: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used — and go! Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen.
Applications
The QIAxcel RNA QC Kit v2.0 provides fast and sensitive analysis of the integrity and quantity of total RNA and cRNA (fragmented or intact).
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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