QIAsymphony RGQ
Integrated automated sample preparation and assay setup,withmanual transfer to the detection unit, for all molecular testing needs
- Unprecedented flexibility
- Workflow optimization and utility
- Maximized ease-of-use and convenience
- Laboratory-developed tests
- Designed for largest range of applications
QIAGEN"spremier sample-to-result workflow solution comprises the QIAsymphony SPfor sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler.QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.
Explore thevirtual demoto learn more about QIAsymphony RGQ.- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:9001850 QIAsymphony RGQ, System Inquire QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor |
Cat No./ID:9020246 QIAsymphony Cabinet SP/AS Inquire Accessory for correct positioning of the QIAsymphony SP/AS instruments |
The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application..
Product Details
QIAsymphony RGQ can save you time by processing up to96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.
QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and ishighly suitedfor laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports laboratory-developed tests for fast implementation of key workflows in your laboratory. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis.
| Kits | Instrument | Method |
|---|---|---|
| QIAsymphony DSP AXpH DNA Kit | QIAsymphony SP | Purification of DNA from liquid-based cytology media |
| QIAsymphony DSP DNA Kits | QIAsymphony SP | Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood |
| QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP | Purification of viral nucleic acids or bacterial DNA from a broad range of sample types |
| QuantiFast Pathogen +IC Kits | QIAsymphony AS | Multiplex real-time PCR and RT-PCR using sequence-specific probes |
| QuantiTect Virus Kits | QIAsymphony AS | Highly sensitive detection of multiple viral DNA and RNA targets in the same tube |
| QuantiTect Probe RT-PCR Kit | QIAsymphony AS | Quantitative, real-time one-step RT-PCR using sequence-specific probes |
| Rotor-Gene Probe RT-PCRKit | QIAsymphony AS | One-step RT-PCR; probe (singleplex) |
| Rotor-Gene MultiplexRT-PCRKit | QIAsymphony AS | PCR and two-step RT-PCR; probe (multiplex) |
The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.
To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferredautomatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See "QIAsymphony workflows".
The QIAsymphony RGQ is the most versatile system for all molecular testing needs— today and tomorrow. The QIAsymphony RGQ allows consolidation of commercial assays, as well as in-house–developed tests, on a single platform.
Future applications will focus on:
- Menu expansion for pathogen detection and genetic testing
- Tests for personalized healthcare
- Pharma co-developed tests
- Tests developed in your lab community
Specifications
Features | Specifications |
| Elution volume | QIAsymphony SP — 30–500 µl (application dependent) |
| Features | Rotor-Gene Q — dynamic range, 10 orders of magnitude |
| Heat dissipation/thermal load | Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour) |
| Humidity | QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C Rotor-Gene Q — relative humidity of 10–75% (noncondensing) |
| Input volume | QIAsymphony SP — minimum input volume: 200 µl (application dependent) |
| Instrument dimensions | QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.) |
| Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit |
| Operating temperature | QIAsymphony SP/AS — 15–32°C (59–89.6ºF) Rotor-Gene Q — 18–30ºC (64–86ºF) |
| Optical System | Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations |
| Overvoltage category | II |
| Place of operation | For indoor use only |
| Pollution level | QIAsymphony SP/AS — 2 Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3) |
| Power | QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse |
| Processing | QIAsymphony SP/AS — automated |
| Protocols/main application on this instrument | QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials QIAsymphony AS — PCR setup Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM) |
| Samples per run (throughput) | QIAsymphony SP — 1–96 samples in batches of 24 Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100 |
| Software | Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD |
| Storage temperature | QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer"s package Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1) |
| Technology | QIAsymphony SP — QIAGEN magnetic-particles chemistry Rotor-Gene Q — real-time PCR cycler |
| Thermal performance | Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling |
| Transportation conditions | QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer"s package Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2) |
| Typical run time | Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent) |
| Warranty | Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs |
| Weight | QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration) |
Product Resources
Customers who bought these products also bought
- Cat No./ID:9018089
Cooling Adapter, Reagent Holder 2, Qsym
Adapter for holding 18 x 2 ml conical tubes, 2 x 5 ml conical tubes, and 2 x Reagent Bottles, 30 ml; for use with the QIAsymphony AS (software version 3.1 or higher)$950.00Add To Cart - Cat No./ID:9242083
Insert, 2.0ml v2, samplecarr. (24), Qsym
Secondary tube adapter (for 2 ml screw-cap tubes, tube insert 3b) for use with the QIAsymphony SP tube carrier$152.00Add To Cart - Cat No./ID:9018092
Cooling Adapter, RG Strip Tubes 72, Qsym
Adapter for holding 18 strips of 4 tubes; for use with the QIAsymphony AS (software version 3.1 or higher)$1,335.00Add To Cart - Cat No./ID:9242057
Tube Insert, 11 mm, Revision, sample carrier, Qsym (24)
Primary tube adapter (11 mm, with tube insert 2A) for use with the QIAsymphony SP tube carrier (all software versions)$248.00Add To Cart - Cat No./ID:9242058
Tube Insert, 13 mm, sample carrier, Qsym (24)
Primary tube adapter (13 mm, with tube insert 1A) for use with the QIAsymphony SP tube carrier (all software versions)$254.00Add To Cart - Cat No./ID:9242204
Adapter 2 x Rotor-Disc, Qsym
Adapter for holding up to 2 x Rotor-Disc 72 on Rotor-Disc 72 Loading Blocks; for use with the QIAsymphony AS (software version 3.1 or higher)$481.00Add To Cart - Cat No./ID:9018937
Adapter, Light Cycler 480 96-Well Plate
For use with the Roche LightCycler 480; 96-well plate; wells hold 10–100 µl; plate has an orientation pin and a “front” indicator$413.00Add To Cart - Cat No./ID:9021670
Adapter, tubes, 2 ml, v2, Qsym
Non-cooling adapter for 2 ml screw-cap tubes (new version; with bar code)$1,765.00Add To Cart - Cat No./ID:9018090
Cooling Adapter, Reagent Holder 1, Qsym
Adapter for holding 18 x 2 ml conical tubes, and 6 x 5 ml conical tubes; for use with the QIAsymphony AS (software version 3.1 or higher)$1,308.00Add To Cart - Cat No./ID:9019873
Carrier, tubes (1), sample track, Qsym
Tube carrier for sample input; for use with the QIAsymphony SP$503.00Add To Cart
