Rotor-Gene SYBR® Green RT-PCR Kit
For ultrafast, one-step qRT-PCR gene expression analysis using SYBR Green I on Rotor-Gene cyclers
- Optimized for ultrafast, reliable results on Rotor-Gene cyclers
- Sensitive detection of even low copy numbers
- Accurate detection of a wide range of template amounts
- Specially formulated, ready-to-use master mix for fast cycling
- Guaranteed performance combined with QuantiTect Primer Assays
The Rotor-Gene SYBR Green RT-PCR Kit is designed for use with the Rotor-Gene Qand other Rotor-Gene cyclers, providing ultrafast, highly specific quantification of RNA targets withreal-time one-step RT-PCR using SYBR Green I detection. Outstanding performance is achieved through the combination of a specially optimized master mix and the unique Rotor-Gene cycler. For convenience, the master mix can be stored at 2–8°C.
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Cat No./ID:204174 Rotor-Gene SYBR Green RT-PCR Kit (400) $961.00 For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2x2ml RNase-Free Water |
Product Details
The Rotor-Gene SYBR Green RT-PCR Kit enables reliable real-time RT-PCR quantification on the Rotor-Gene Q without the need for optimization of reaction and cycling conditions. Real-time one-step RT-PCR is carried out, which means that RNA is used as template in a reaction where reverse transcription and PCR take place sequentially in the same reaction vessel. Since it is not necessary to transfer the finished RT reaction to another tube for PCR, the real-time RT-PCR procedure is streamlined, making high-throughput analysis possible.
The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. Highly specific amplification is assured through a balanced combination of K+ and NH4+ ions, which promote specific primer annealing, enabling high PCR specificity and sensitivity (see figure "Specific primer annealing"). Fast cycling without compromising performance is achieved using Q-Bond, a novel PCR additive that considerably shortens cycler run times (see figure "Fast primer annealing").
| Component | Features | Benefits |
|---|---|---|
| HotStarTaq Plus DNA Polymerase | 5 min activation at 95ºC | Set up of qPCR reactions at room temperature |
| Rotor-Gene SYBR Green RT-PCR Buffer | Balanced combination of NH4+ and K+ ions | Specific primer annealing ensures reliable qPCR results |
| Unique Q-Bond additive | Faster PCR run times enable faster results and more reactions per day | |
| SYBR Green I dye | Yields a strong fluorescent signal upon binding double-stranded DNA | Highly sensitive quantification |
| Rotor-Gene RT Mix | Special blend of reverse transcriptases with a high affinity for RNA | RNA can be transcribed in just 10 minutes, even through complex secondary structures |
A ready-to-use master mix eliminates the need for optimization of reaction and cycling conditions. Simply add template RNA, primers, and the supplied reverse transcriptase mix to the master mix and program the cycler. Instructions are provided in the detailed handbook supplied with the kit.
For gene expression analysis using real-time one-step RT-PCR, the combination of the Rotor-Gene SYBR Green RT-PCR Kit with QuantiTect Primer Assays and the Rotor-Gene Q provides a complete, ready-to-run solution. QuantiTect Primer Assays are bioinformatically validated primer sets for any gene from human, mouse, rat, and many other species. Assays can be easily ordered online at the GeneGlobe Web portal.
The Rotor-Gene SYBR Green RT-PCR Kit provides rapid real-time quantification of RNA targets on the Rotor-Gene Q. The kits are also compatible with the Rotor-Gene 3000 and the Rotor-Gene 6000.
Specifications
Features | Specifications |
| Applications | Real-time quantification of RNA targets |
| Description | For ultrafast quantitative real-time one-step RT-PCR using SYBR Green I |
| Reaction type | Real-time one-step RT-PCR |
| Real-time or endpoint | Real-time |
| Sample/target type | RNA |
| Single or multiplex | Single |
| SYBR Green I or sequence-specific probes | SYBR Green I |
| Thermal cycler | Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000 |
| With or without ROX | Without ROX dye |
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